Primjena Diversity Arrays tehnologije (DArT) i sekvenciranja nove generacije u genotipizaciji hrvatskih kultivara graha – principi i metode

undergraduate thesis

• Title (english): Application of Diversity Arrays technology (DArT) and new generation sequencing in the genotyping of Croatian bean cultivars - principles and methods
• Author: Nikolina Valjak
• Mentor: Zlatko Liber (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cilj ovog završnog seminara je bio prikazati osnovne principa metoda genotipizacije, razmatranih i primjenjivanih prilikom genotipizacije hrvatskih kultivara graha unutar projekta: „Genetska osnova količine bioaktivnih hranivih tvari hrvatskih tradicijskih kultivara graha“, financiranog od strane Hrvatske zaklade za znanost. U seminarskom radu su opisane tradicionalne metode genotipizacije kao što su AFLP, mikrosateliti i SNP biljezi. Budući da se DArTseq metoda pokazale najpogodnijim za dostizanje glavnih ciljeva projekta, osobita pažnja je posvećena metodama temeljenim na sekvenciranju nove generacije (eng. Next Generation Sequencing, skraćenica NGS).

Abstract (english)

The aim of this final seminar was to present the basic principles of the methods of genotyping that were considered and applied during the genotyping of Croatian bean cultivars within the project: "Genetic basis of bioactive nutrient content in Croatian common bean landraces", funded by the Croatian Science Foundation. The traditional methods of genotyping such as AFLP, microsatellite and SNP markers were described. Since the DArTseq method has proved to be the most suitable for reaching the main project objectives, special attention was paid to the Next Generation Sequencing (NGS) methods.

• Keywords: genotipizacija; hrvatski kultivari graha
• Keywords (english): genotyping; Croatian bean cultivars
• Language: croatian
• URN:NBN: urn:nbn:hr:217:661875
• Study programme: Title: Environmental Sciences; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) struke znanosti o okolišu (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-10-19 07:54:31