Upotrebljivost gena za ribosomsku RNA i gena koji kodiraju proteine u rekonstrukciji filogenetskih odnosa unutar porodice Elmidae (Coleoptera)

master's thesis

• Title (english): Phylogenetic utility of ribosomal and protein-coding genes for reconstructing the phylogeny of Elmidae (Coleoptera)
• Author: Tin Tomaš
• Mentor: Branka Bruvo Mađarić (mentor)
• Committee member: Marko Močibob (predsjednik povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Tomica Hrenar (član povjerenstva)
• Committee member: Ivan Kodrin (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2017-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Elmidae (Coleoptera) je porodica vodenih kornjaša koji se često koriste kao bioindikatori u
tekućicama, no filogenetski odnosi unutar porodice su dosada slabo istraženi. U ovom radu
analizirana je upotrebljivost gena za ribosomsku RNA i odabranih gena koji kodiraju proteine
za pouzdanu rekonstrukciju filogenetskih odnosa vrsta, rodova i podporodica unutar porodice
Elmidae. Filogenetske analize pokazale su da su geni za COI i 12S rRNA vrlo varijabilni, a
gen za 18S rRNA je izuzetno konzerviran te ovi markeri ne daju pouzdane rezultate.
Nuklearni geni za 28S rRNA, CAD i Arg daju bolje razlučivanje i podršku za topologiju.
Kombiniranjem većeg broja markera postignuti su znatno bolji rezultati, posebice
kombinacijom nuklearnih gena. U većini analiza podporodice Elminae i Larainae su
međusobno parafiletske, te ovo istraživanje upućuje na potrebu revizije taksonomskog statusa
skupina unutar porodice Elmidae.

Abstract (english)

Elmidae (Coleoptera) is a family of water beetles which are often used as bioindicators
in running waters. The phylogenetic relationships within the family are not very well studied.
In this study we analysed the usefulness of ribosomal RNA coding genes and protein coding
genes in the reconstruction of phylogenetic relationships within Elmidae. Phylogenetic
analyses of COI and 12S rRNA genes have shown that these genes have to much variability,
while the gene for 18S rRNA is too conserved to give reliable results. Nuclear genes for 28S
rRNA, CAD and ArgK give substantially better results. The best results were obtained by
combinig different genes, especially nuclear genes. In most analyses subfamilies Elminae and
Larainae are shown to be paraphyletic. This study shows the need for revising the taxonomic
status of taxa within the family Elmidae.

• Keywords: Elmidae; Coleoptera; molekularna filogenetika; molekularni markeri
• Keywords (english): Elmidae; Coleoptera; molecular phylogenetics; molecular markers
• Language: croatian
• URN:NBN: urn:nbn:hr:217:268994
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-10-23 14:14:22