Učinkovitost popravka DNA u prirodnim populacijama dagnje Mytilus galloprovincialis

doctoral thesis

• Title (english): DNA repair capacity in natural mussel Mytilus galloprovincialis population
• Author: Mirta Smodlaka; MBZ: 318536
• Mentor: Maja Fafanđel (mentor); MBZ: 218864
• Committee member: Nevenka Bihari (predsjednik povjerenstva)
• Committee member: Renato Batel (član povjerenstva); MBZ: 92412
• Committee member: Mirjana Pavlica (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Geology); Zagreb
• Defense date and country: 2015-06-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Interdisciplinary Natural Sciences; Marine Science
• Universal decimal classification (UDC): 55 - Geology. Meteorology. Hydrology ; 575 - General genetics. General cytogenetics ; 591.2 - Animal pathology. Diseases of undomesticated animals

Abstract

Popravak DNA je ključni mehanizam održavanja integriteta genoma i obrane od posljedica učinka genotoksičnih zagađivala. Sposobnost i brzina popravka predstavljaju važan biomarker za praćenje kvalitete morskog okoliša i postoji potreba za jednostavnom i ekonomičnom metodom mjerenja učinkovitosti popravka DNA u bioindikatorskim vrstama. Optimizacijom uvjeta za fluorimetrijsku detekciju 3D metodom u ovom radu je uspostavljen protokol za mjerenje učinkovitosti popravka DNA u probavnoj žlijezdi i škrgama dagnje Mytilus galloprovincialis. Utvrđeno je da učinkovitost popravka DNA u probavnoj žlijezdi dagnje je viša nego u škrgama, što ukazuje na tkivnu specifičnost popravka DNA, a njihova međusobna korelacija ukazuje na usklađeni odgovor ovih organa na prisutnost genotoksina. Integritet DNA u hemocitima dagnje Mytilus galloprovincialis, kao pokazatelj genotoksičnog pritiska okoliša, korelira s učinkovitosti popravka DNA probavne žlijezde, koja se razlikuje za populacije dagnje koje naseljavaju područja s različitim antropogenim pritiskom. Zaključuje se da je 3D metodom moguće sustavno praćenje genotoksičnog rizika u prirodnim populacijama dagnje, čime se može unaprijediti procjena genotoksičnog rizika u ekotoksikološkim studijama.

Abstract (english)

DNA repair is a key mechanism for the genome integrity maintenance and defense against the consequences of genotoxic pollutants. The DNA repair capacity in marine bioindicator species is an important biomarker for the marine environment monitoring that demand a simple and economic method. Following optimization of the 3D method a new protocol was established that allows a fluorimetric measurement of the DNA repair capacity in the digestive gland and gills of mussel Mytilus galloprovincialis was tested. The DNA repair capacity in the digestive gland was higher than in the gills suggesting that DNA repair capacity is tissue specific. The correlation between the DNA repair capacities of investigated tissues indicates a coordinated response of the organism to genotoxic pressure. DNA repair capacity in digestive gland distinguished population living under different anthropogenic pressure as well as correlated with the DNA integrity in haemocytes as indicators of environmental genotoxic pressure. Introduction of the 3D method measurement of DNA repair capacity in to the ecotoxicological studies could improve the assessment of the genotoxic risks in the marine environment.

• Keywords: Popravak DNA; cjelovitost DNA; genotoksičnost; dagnja; 3D metoda; protočna citometrija; Jadransko more
• Keywords (english): DNA repair; DNA integrity; genotoxicity; mussel; 3D assay; flow cytometry; Adriatic Sea
• Language: croatian
• URN:NBN: urn:nbn:hr:217:185341
• Project: Number: 098-0982705-2725; Title: Ekotoksični učinci onečišćenja na morske organizme; Leader: Nevenka Bihari; Jurisdiction: Croatia; Funder: MZOS
• Study programme: Title: Interdisciplinary doctoral study in Oceanology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje interdisciplinarne prirodne znanosti (znanost o moru) (doktor/doktorica znanosti, područje prirodnih znanosti, polje interdisciplinarne prirodne znanosti (znanost o moru))
• Type of resource: Text
• Extent: 108 (+XI) str. ; 32 cm
• File origin: Born digital
• Access conditions: Open access
• Public note: Ovaj je doktorski rad izrađen u Laboratoriju za morsku ekotoksikologiju Centra za istraživanje mora Instituta Ruđer Bošković u Rovinju, u sklopu navedenog studija. Rad je opremljen sredstvima Centra za istraživanje mora Instituta Ruđer Bošković u Rovinju i Ministarstva znanosti, obrazovanja i sporta Republike Hrvatske u programu Prirodni i antropogeni utjecaji na procese i ekološke odnose u Jadranskom moru u sklopu navedenog projekta.
• Created on: 2017-02-16 11:07:12