Aktivnost kisele deoksiribonukleaze i histokemijske promjene u lizosomima kao odgovor dagnje Mytilus galloprovincialis na čimbenike u okolišu

doctoral thesis

• Title (english): Acid deoxyribonuclease activity and histochemical perturbation of mussel Mytilus galloprovincialis lysosomes in response to environmental conditions
• Author: Ines Kovačić; MBZ: 317186
• Mentor: Maja Fafanđel (mentor); MBZ: 218864
• Committee member: Renato Batel (predsjednik povjerenstva); MBZ: 92412
• Committee member: Mauro Štifanić (član povjerenstva); MBZ: 251681
• Committee member: Damir Kapetanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Geology); Zagreb
• Defense date and country: 2015-04-07, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Interdisciplinary Natural Sciences; Marine Science
• Universal decimal classification (UDC): 55 - Geology. Meteorology. Hydrology

Abstract

Kisela DNaza je lizosomalni enzim koji ima važnu ulogu u metabolizmu deoksiribonukleinskih kiselina. Aktivnost kisele DNaze utvrđena je u probavnoj žlijezdi i škrgama dagnje Mytilus galloprovincialis. Kao dominantna DNaza, identificirana je i karakterizirana DNaza II koja je lokalizirana u lizosomu. Utvrđene promjene aktivnosti kisele DNaze u probavnoj žlijezdi povezane su s dostupnosti hrane u okolišu i ciklusom razmnožavanja, dok je aktivnost kisele DNaze u škrgama povezana sa sezonskim promjenama temperature i saliniteta. Aktivnost kisele DNaze u oba organa u dagnjama na onečišćenoj postaji razlikuje se od aktivnosti u dagnjama s referentne postaje. Integracija aktivnosti kisele DNaze u probavnoj žlijezdi s histokemijski detektiranim lizosomalnim promjenama doprinijela je boljem razdvajanju odgovora obzirom na onečišćenje. Podatci koji se odnose na aktivnost kisele DNaze u probavnoj žlijezdi predstavljaju dobru osnovu za buduća istraživanja koja uključuju integrirani odgovor lizosoma prilikom procjene stanja okoliša.

Abstract (english)

Acid DNase is lysosomal enzyme that plays an important role in the deoxyribonucleic acid metabolism. DNase II was identified and characterized in digestive gland and gills lysosomes of mussel Mytilus galloprovincialis as the dominant and representative acid DNase. Acid DNase activity in the digestive gland was associated with food availability and reproduction cycle, while the acid DNase activity in the gills was associated with seasonal changes in seawater temperature and salinity. Acid DNase activity in both investigated organs differs between mussels from contaminated and reference sampling site. Integration of digestive gland acid DNase activity and histochemical perturbation of lysosomes enabled better differentiation of sampling station due to contamination. Observed acid DNase activity in the digestive gland represents a good basis for future environmental monitoring studies that would include integrated lysosomal response.

• Keywords: kisela DNaza; DNaza II; odgovor lizosoma; školjkaši
• Keywords (english): acid DNase; DNase II; lysosomal responce; mussel
• Language: croatian
• URN:NBN: urn:nbn:hr:217:486363
• Study programme: Title: Interdisciplinary doctoral study in Oceanology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje interdisciplinarne prirodne znanosti (znanost o moru) (doktor/doktorica znanosti, područje prirodnih znanosti, polje interdisciplinarne prirodne znanosti (znanost o moru))
• Type of resource: Text
• Extent: 88 (+X) str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Public note: Doktorska disertacija izrađena je u Centru za istraživanje mora Instituta Ruđer Bošković u Rovinju, u sklopu navedenog studija.
• Created on: 2017-02-23 08:29:09