Toksikodinamika herbicida prometrina u slezeni miša

master's thesis

• Title (english): Toxicodynamics of promethryne herbicide in mouse spleen
• Author: Marija Majer
• Mentor: Petar-Oskar Springer (mentor)
• Committee member: Petar-Oskar Springer (predsjednik povjerenstva)
• Committee member: Mirjana Kalafatić (član povjerenstva)
• Committee member: Sven Jelaska (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2008, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U ekološkim sustavima antropogenim su djelovanjem unešeni raznoliki herbicidi, u pravilu štetni i za zdravlje ljudi. Prometrin se ubraja u metiltiotriazinske herbicide, malo je do umjereno toksičan spoj i selektivni pesticid opće namjene. Njegovi su imunotoksični učinci do sada nedovoljno istraženi, a toksikodinamika nejasna. Slezena je kao dio imunološkog sustava meta imunotoksičnog djelovanja pesticida i nekih karcinogena, i stoga često ciljni organ u toksikološkim studijama. Kako bi se utvrdila koncentracija prometrina, nakon žrtvovanja i razudbe tretiranih životinja uzorci slezene analizirani su metodom vezanog sustava plinske kromatografije-spektrometrije masa. Utvrđena je realna bioraspoloživa koncentracija prometrina kojoj su izloženi splenociti tijekom 1, 6, 24 i 48 sati. Zaključak je da slezena s obzirom na akutnu unešenu dozu od 375 mg kg-1 (1/10 LD50) nema bioakumulativni potencijal za prometrin. Razlike među spolovima nisu vidljive u svim kinetičkim fazama, no upućuju na razlike u metaboliziranju prometrina ovisno o spolu. Rezultati toksikodinamike prometrina u slezeni mogu se neposredno koristiti za procjenu rizika alometrijskim metodama usporedbom na drugim vrstama i čovjeku.

Abstract (english)

A variety of herbicides is discharged into ecological systems as a consequence of human activity. Promethryne is one of the methiltiotriazine herbicides. This selective pesticide of common use is slightly to moderately toxic. It's immunotoxic effects and toxicodynamics have been poorely studied so far. Spleen is one of the target sites of immunotoxic effects caused by pesticides or some carcinogens. Gas chromatography-mass spectrometry assay was applied to detect promethryne concentrations in spleen samples of acutely treated mice after 1st, 6th, 24th and 48th hour post exposure. Bioavailable concentrations of promethryne in spleen were indentified. The spleen bioaccumulation potential was not significant after single oral 375 mg kg-1 (1/10 LD50) dose. Sex differences could not be detected during each kinetic phase, but do indicate sex dependent differences in promethryne metabolic processing. Results of promethryne toxicodynamics analysis in spleen can be applied to risk assessment via alometric scaling from animal to humans.

• Keywords: herbicidi; toksikodinamika prometrina; slezena; alometrijske metode; procjena rizika
• Keywords (english): herbicides; toxicodynamics of promethryne; spleen; alometric scaling; risk assessment
• Language: croatian
• URN:NBN: urn:nbn:hr:217:283081
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-05 12:12:09