Toksičnost komercijalnih surfaktanata na kvasac Saccharomyces cerevisiae

master's thesis

• Title (english): The toxicity of commercial surfactants on yeast Saccharomyces cerevisiae
• Author: Vlatka Brajak
• Mentor: Jasna Hrenović (mentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Mirjana Kalafatić (član povjerenstva)
• Committee member: Zoran Tadić (član povjerenstva)
• Committee member: Sven Jelaska (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cilj ovog rada je utvrđivanje toksičnosti komercijalnih surfaktanata kao sastavnog dijela deterdženata, omekšivača rublja i sredstava za čišćenje koji se koriste u domaćinstvima. Ispitano je 15 komercijalnih surfaktanata. Za utvrđivanje toksičnosti korištena su dva testa na kvascu S. cervisiae, fermentacijski test i test metilenskog modrila. Dobivene su EC50 vrijednosti prema kojima su surfaktanti svrstani u tri kategorije štetnosti. Tri ispitana surfaktanta pokazala su se toksičnim s izmjerenim EC50 vrijednostima između 1 i 10 mg/L, dok je šest spojeva pokazalo vrlo nisku toksičnost, s izmjerenim EC50 vrijednostima znatno iznad 100 mg/L. Ostalih šest uzoraka svrstano je u kategoriju štetnih s izmjerenim EC50 vrijednostima između 10 i 100 mg/L. Test metilenskim modrilom na kvascu S.cerevisiae pokazao se osjetljivijim od fermentacijskog testa jer je uglavnom davao niže EC50 vrijednosti.

Abstract (english)

The goal of this study was to assess toxicity of commercial surfactants as essential parts of detergent, fabric softeners and cleaning products which are used in households. 15 commercial surfactants were analyzed. The toxicity was evaluated by two tests on yeast, S. cervisiae, fermentation test and methylene blue dye test. According to obtained EC50 values sufractants were sorted in three categories of adversity. Three tested sufractants were proven toxic with EC50 values ranking between 1 and 10 mg/L, while six compounds showed very low level of toxicity with EC50 values considerably higher than 100 mg/L. Remaining six samples had been classified as not harmful for the enviroment, as their EC50 values ranked between 10 and 100 mg/L. Methylene blue dye test on yeast S. cervisiae was found to be more sensitive then fermentation test, becouse the estimated EC50 values using methylene blue dye test were mainly lower.

• Keywords: deterdžent; testovi toksičnosti; S. cerevisiae; fermentacija; metilensko modrilo; postotak inhibicije; efektivna koncentracija EC50
• Keywords (english): detergent; toxicity tests; S. cervisiae; fermentation; methylene blue; inhibition percentage; effective concentration EC50
• Language: croatian
• URN:NBN: urn:nbn:hr:217:681454
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-07 09:11:38