Učinak flavonoida na frekvenciju mikronukleusa u krvi miševa s dijabetesom

master's thesis

• Title (english): Effect of flavonoids on micronuclei frequecy in blood of diabetic mice
• Author: Antonia Goričanec
• Mentor: Nada Oršolić (mentor)
• Committee member: Mirjana Kalafatić (predsjednik povjerenstva)
• Committee member: Mirta Tkalec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Mikronukleus testom istražili smo mogući antidijabetički učinak pripravaka epigalokatehin galata (EGCG-a) i flavonoida propolisa (kvercetina, naringina, naringenina i krizina) u miševa s dijabetesom. Tip 1 dijabetesa induciran je u miševa injekcijom aloksana u dozi od 75 mg/kg iv. Drugog dana nakon injiciranja dolazi do značajnog povećanja razine glukoze u krvi, u odnosu na kontrolnu skupinu miševa neobrađenih aloksanom. Dnevnom obradom miševa flavonoidima i EGCG-om u dozi od 50 mg/kg, tijekom 7 dana, zabilježeno je poboljšanje općeg stanja životinja s dijabetesom. Primjenom EGCG-a / flavonoida u slučajevima ranog dijabetesa dolazi do znatno povećanog broja mikronuleusa u retikulocitima periferne krvi u odnosu na dijabetične životinje bez obrade. Obrada dijabetičnih životinja naringeninom ili kvercetinom u kasnom dijabetesu dovela je do smanjenja broja mikronukleusa u retikulocitima u odnosu na neobrađene životinje s dijabetesom; dok obrada s EGCG-om pokazuje neznatni porast broja mikronukleusa. Istraživanja doza-ovisnog učinka, kao i razine antioksidativnih enzima, pridonijeti će boljem poznavanju učinkovitosti flavonoida i EGCG-a u smanjivanju posljedica dijabetesa. Neki od rezultata pokazuju obećavajući antidijabetički učinak flavonoida / EGCG-a.

Abstract (english)

Using micronucleus test we investigated the possible antidiabetic effects of epigallocatechin gallate (EGCG) and flavonoids derived from propolis (quercetin, naringin, naringenin and chrysin) in diabetic mice. Type 1 diabetes was induced in mice by injection of alloxan in dose of 75 mg/kg iv. Two days after injection the level of blood glucose was representatly increased compared to healty mice group without alloxan treatment. With daily intake of EGCG and flavonoids in dose of 50 mg/kg during 7 days the general status of diabetic animals was improved. Treatment of mice with EGCG/flavonoids in cases of early diabetes increased the number of micronuclei in peripheral blood reticulocytes compared to diabetic animals without treatment. Number of micronuclei in mice treated with naringenin, quercetin in later diabetes was decreased in relation to nontreated diabetic animals, while treatment of mice with EGCG slightly increased the number of micronucleus. Research of dose-dependent effect, as well as antioxidative enzymes, are contributing better understanding of flavonoid and EGCG efficiency in reducing outcomes of diabetes. Some of the results show promising antidiabetic effect of flavonoids/EGCG.

• Keywords: dijabetes; oksditavni stres; antioksidansi; flavonoidi; mikronukleus test
• Keywords (english): diabetes; oxidative stress; antioxidants; flavonoids; micronucleus test
• Language: croatian
• URN:NBN: urn:nbn:hr:217:201127
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-08 11:24:07