Raznolikost haplotipova DQA/DQB/DRB glavnog sustava tkivne podudarnosti skupine II u populaciji čagljeva (Canis aureus) u Srbiji

master's thesis

• Title (english): Variability of major histocompatibility complex class II DQA/DQB/DRB haplotypes in the golden jackal (Canis aureus) from Serbia
• Author: Veronika Lončar
• Mentor: Ana Galov (mentor)
• Mentor: Haidi Arbanasić (pomoć pri izradi, neposredni voditelj)
• Committee member: Ana Galov (predsjednik povjerenstva)
• Committee member: Sunčica Bosak (član povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Committee member: Renata Matoničkin Kepčija (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-02-21, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Glavni sustav tkivne podudarnosti (MHC) sudjeluje u adaptivnom imunosnom odgovoru sisavaca. Veća raznolikost gena MHC omogućuje limfocitima T da prepoznaju širi spektar antigena, što u konačnici daje jedinci, ali i populaciji veću šansu za preživljavanje napada patogena. Dobar su molekularni marker za proučavanje genetičke raznolikosti vrsta i populacija zbog svoje poligenosti i polimorfnosti. Čagalj (Canis aureus) je zvijer (red Carnivora) koja pripada porodici pasa (Canidae). Istraživanja raznolikosti gena MHC kod čaglja tek su nedavno započela unatoč njegovoj širokoj rasprostranjenosti na euroazijskom i afričkom kontinentu. Cilj ovog istraživanja je određivanje raznolikosti tro-lokusnih haplotipova DQA/DQB/DRB te pojedinih lokusa skupine II sustava MHC u populaciji čagljeva iz Srbije. U ovom istraživanju na 29 uzoraka mišića čagljeva pronađeno je tri alela lokusa DQA i DQB, četiri alela lokusa DRB te šest haplotipova. Sedam je alela dosad pronađeno samo kod čaglja, a tri su trans-specijski polimorfizmi. Tri su haplotipa pronađena samo u srpskoj populaciji, a tri haplotipa su zajednička s istočnoeuropskom populacijom. Potvrđena je postavljena hipoteza djelovanja pozitivne selekcije na svim lokusima koristeći dN/dS test, odnosno omjer nesinonimnih i sinonsimnih supstitucija na nukleotidnom slijedu alela.

Abstract (english)

The genes of the major histocompatibility (MHC) system takes part in the adaptive immune response in mammals. Greater diversity of MHC genes enables T lymphocytes to recognise a wider spectrum of antigens, which in turn gives the individual a greater chance of surviving a pathogen attack. They are a good molecular marker for studying genetic diversity of both species and populations because they are polygenic and polymorphic. The golden jackal (Canis aureus) is a carnivore from the dog family (Canidae). Studies of MHC gene diversity of golden jackals have only recently emerged despite its wide distribution on the Eurasian and African continent. The aim of this graduate thesis is to determine variability of three-locus DQA/DQB/DRB haplotypes and individual Class II MHC loci in golden jackals from Serbia. In this study of 29 jackal muscle samples we have found three DQA and DQB alleles, four DRB alleles and six haplotypes. Seven alleles have only been found in jackals so far, while three alleles are trans-species polymorphisms. Three haplotypes are private to the serbian populations, while three haplotypes are shared with the Eastern European population. We have confirmed the proposed hypothesis of existing positive selection on all loci using the dN/dS test, i.e. the ratio of non-synonymous and synonymous substitutions on the allelic nucleotide sequences.

• Keywords: MHC; Canis aureus; DQA; DQB; DRB; haplotip
• Keywords (english): Major histocompatibility complex; Canis aureus; balancing selection haplotypes
• Language: croatian
• URN:NBN: urn:nbn:hr:217:476730
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-14 11:44:57