Zakorjenjivanje i aklimatizacija crnoplodne aronije (Aronia melanocarpa Michx.) razmnožene u uvjetima in vitro

master's thesis

• Title (english): Rooting and acclimatization of black chokeberry (Aronia melanocarpa Michx.) shoots
• Author: Silvija Črnjević
• Mentor: Branka Pevalek-Kozlina (mentor)
• Mentor: Marija Babić (pomoć pri izradi, neposredni voditelj)
• Committee member: Branka Pevalek-Kozlina (predsjednik povjerenstva)
• Committee member: Dubravka Matković-Čalogović (član povjerenstva)
• Committee member: Biserka Prugovečki (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Crnoplodna aronija (Aronia melanocarpa Michx.) je listopadni grm iz porodice Rosaceae. Zbog izvanrednih antioksidacijskih svojstava plodova, vrsta je uvedena u kulturu in vitro. U ovom radu prikazani su rezultati istraživanja zakorjenjivanja i aklimatizacije crnoplodne aronije na hranjivoj podlozi MS s različitim koncentracijama makroelemenata (MS, ½ MS i ¼ MS). Najveća stopa zakorjenjivanja (85,4%) te najbolje morfološke značajke biljaka postignute su na podlozi ½ MS. Istraživanje je nastavljeno na ½ MS uz dodatak auksina α- naftalenoctene kiseline (NAA) i indol-3-maslačne kiseline (IBA). Dodani auksini inhibirali su stvaranje primarnog te potaknuli stvaranje adventivnog korijenja iz prethodno nastalog kalusnog tkiva. Na podlozi s 1,5 mg/L NAA stopa adventivnog zakorjenjivanja iznosila je 95,8%, a na podlozi s 1,0 mg/L IBA postotak adventivnog zakorjenjivanja iznosio je 100%. Aklimatizacija in vitro zakorjenjenih biljaka bila je podjednako uspješna (u 80-90% biljka) na komercijalnom (COMPO SANA) i na pripremljenom supstratu (mješavina pumice, tenisit i treseta u omjeru 3:3:1). Zakorjenjivanje izdanaka ex vitro provedeno je na istim supstratima uz istovremenu aklimatizaciju, te se pokazalo manje uspješnim (u 50-60% biljaka) od aklimatizacije biljaka prethodno zakorjenjenih u uvjetima in vitro (u 80-90% biljka).

Abstract (english)

Black chokeberry (Aronia melanocarpa Michx.) is a deciduous bush-like plant which belongs to the Rosaceae family. The species was introduced in in vitro culture due to high atioxidative activity of their fruits. Rooting and subsequent acclimatization of black chokeberry has been investigated on MS nutrient media containing different concentration of macronutrients (MS, ½ MS, ¼ MS). The highest rooting percentage (85.4%) and the best morphological characteristics were obtained on ½ MS medium. Therefore, the research was continued on ½ MS, supplemented with auxins α- naphthaleneacetic acid (NAA) or indole-3-butyric acid (IBA). Auxins NAA or IBA inhibited the main root induction, but stimulated adventitious root formation from the callus. Maximum adventitious rooting occured in shoots grown on ½ MS containing 1.5 mg/L NAA (95.8%) or 1.0 mg/L IBA (100%). Acclimatization of in vitro rooted microcuttings was equally successful (80-90%) in plants potted to commercial peat substrate (COMPO SANA) and mixture of pumice, tenisit and peat (3:3:1). Aronia melanocarpa microcuttings without roots exhibited poor ex vitro rooting and low acclimatization percentage (50-60%).

• Keywords: Aronia melanocarpa; mikropropagacija; MS; zakorjenjivanje; aklimatizacija
• Keywords (english): Aronia melanocarpa; micropropagation; MS; rooting; acclimatization
• Language: croatian
• URN:NBN: urn:nbn:hr:217:690919
• Study programme: Title: Biology and Chemistry; Study programme type: university; Study level: graduate; Academic / professional title: profesor biologije i kemije (profesor biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-21 08:53:24