Selektivnost komercijalnih krutih medija za izolaciju ukupnih koliformnih bakterija iz površinskih voda

master's thesis

• Title (english): Selectivity of commercial solid media for isolation of total coliform bacteria from surface water
• Author: Senka Šimunović
• Mentor: Jasna Hrenović (mentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Zlatko Mihaljević (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Committee member: Davor Kovačević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Istraživanje selektivnosti komercijalnih krutih medija za izolaciju ukupnih koliformnih bakterija iz površinskih voda provedeno je na uzorku čiste kulture vrste Escherichia coli i 19 uzoraka vode prikupljenih iz 7 potoka grada Zagreba, jezera Botaničkog vrta i pet maksimirskih jezera. Uzorci su nacijepljeni na istraživane medije metodom širenja razmaza. Cilj ovog rada bio je istražiti učinkovitost izolacije ukupnih koliformnih bakterija i vrste Escherichia coli na četiri komercijalna kruta medija: EC-X Gluc agar, Endo agar, m-Faecal Coliform agar i MacConkey agar. Uspoređujući podatke dobivene brojanjem izraslih kolonija koliformnih bakterija ustanovljeno je da se mediji razlikuju po broju izraslih kolonija. MacConkey agar je medij na kojemu je izraslo najviše kolonija te je to ujedno i najbolji medij za izolaciju ukupnih koliformnih bakterija. Slijede ga Endo agar i m-Faecal Coliform agar koji se jako malo razlikuju u rezultatima. Najlošija podloga za izolaciju ukupnih koliformnih bakterija je EC-X Gluc agar. Kako su mediji diferencijalni, posebno su pobrojane i kolonije vrste Escherichia coli. Najselektivniji podloga za izolaciju vrste Escherichia coli je, prema dobivenim rezultatima, MacConkey agar. Slijede ga m-Faecal Coliform agar i Endo agar. Najlošija podloga za izolaciju vrste Escherichia coli je EC-X Gluc agar.

Abstract (english)

Selectivity of commertial media for isolation of total coliform bacteria from surface water, was investigated on sample of clean culture of Escherichia coli and 19 samples of water, collected at seven brooks of Zagreb, at lake of Botanical Garden and five lakes of Maksimir. Samples was inoculated on investigated media with Plate Strake Method. The aim of this research was to investigate efficiency of isolation of total coliform bacteria and Escherichia coli on four commertial solid media: EC-X Gluc agar, Endo agar, m-Faecal Coliform agar and MacConkey agar. The data comparison established that the media are different by the number of grown colonies. MacConkey agar is media where the most colonies were grown, this is at the same time the best media for isolation of total coliform bacteria. The following two are Endo agar and m-Faecal Coliform agar, which results are not that different. The worst medium for isolation of coliform bacteria is EC-X Gluc agar.Because the media are differential, the colonies of Escherichia coli were separately counted. The results show that the most selective medium for isolation of Escerichia coli is MacConkey agar. It's followed by m-Faecal Coliform agar and Endo agar. The worst medium for isolation of Escerichia coli is EC-X Gluc agar.

• Keywords: koliformne bakterije; Escherichia coli; hranjive podloge
• Keywords (english): coliform bacteria; Escherichia coli; solid media
• Language: croatian
• URN:NBN: urn:nbn:hr:217:154892
• Study programme: Title: Biology and Chemistry; Study programme type: university; Study level: graduate; Academic / professional title: profesor biologije i kemije (profesor biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-21 09:55:38