Socijalno i emotivno ponašanje štakora nakon perinatalne primjene prekursora serotonina

master's thesis

• Title (english): Social and emotional behaviour in rats after perinatal treatment with serotonin precursor
• Author: Lejla Čolić
• Mentor: Dubravka Hranilović (mentor)
• Committee member: Antun Alegro (predsjednik povjerenstva)
• Committee member: Davor Zanella (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2010, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Serotonin (5- hidroksitriptamin, 5HT) je biološki aktivan amin. Nalazi se u mozgu i na periferiji gdje vrši različite fiziološke uloge. 5HT također kontrolira razvoj mozga. Promjene u sustavu koji regulira metabolizam i funkciju 5HT mogle bi predstavljati jednu od mogućih bioloških podloga poremećaja ponašanja u koje spada i autizam. Smatra se da visoka razina 5HT u krvi, u doba fetalnog i ranog postanatalnog razvoja, može inhibirati rast i razvoj 5HT neurona i tako dovesti do anatomskih i funkcionalnih promjena u mozgu. Kako bi se ispitale posljedice visoke razine 5HT u krvi u doba perinatalnog razvoja na središnji odjeljak 5HT, štakori su bili tretirani prekursorom 5HT, 5- hidroksitriptofanom (5HTP, 25mg/kg ), od 13. gestacijskog dana do 21. postnatalnog dana života. U ovom radu smo istražili posljedice perinatalnog tretmana prekursorom serotonina na kognitivno, socijalno i emotivno ponašanje štakora. Eksperimentalne štakore tretirane 5HPT-om i kontrolne štakore tretirane fiziološkom otopinom podvrgnuli smo bateriji testova ponašanja kojima smo redom ispitali: učenje i kognitivnu rigidnost, lokomotorno ponašanje, anksioznost i eksploratorno ponašanje, socijabilnost te reakciju i habituaciju na opetovani zvučni podražaj. Skupina tretirana 5HPT-om pokazala je povećanu lokomotronu aktivnost i anksioznost, te smanjenu socijabilnost i habituaciju na zvuk visoke frekvencije u odnosu na kontrolnu skupinu. Sposobnost učenja i kognitivna rigidnost nisu se razlikovale između skupina. Rezultati pokazuju da je perinatalni tretman prekursorom serotonina utjecao na ponašanja regulirana serotoninom, što ukazuje na promjene u središnjem odjeljku 5HT nastale tijekom razvoja mozga.

Abstract (english)

Serotonin (5- hydroxytryptamine, 5HT) is a biologically active amine present in both, brain and peripheral tissues where it serves different physiological functions. 5HT also regulates brain development. Disruption of 5HT metabolic pathway or impairments in its function could represent a base for different bahavioural disorders including autism. During prenatal and early postnatal development, high concentrations of serotonin in blood could inhibit 5HT neuronal growth and differentiation, and so cause anatomical and functional changes in brain. To examine consequences of high blood 5HT concentrations during perinatal development, rats were treated with 5HT precursor, 5HTP (5- hydroxytryptophan, 25mg/kg ) from 13th gestational day till 21st postnatal day. In this study we explored consequences of the mentioned perinatal treatment on cognitive, social and emotional behaviour in rats. Experimental group (treated with 5HTP) and control group (treated with saline solution) of rats were submitted to the battery of tests. We examined learning and cognitive rigidity, locomotor activity, anxiety and exploratory behaviour, sociability, and reaction and habituation to repeated high-frequency sound. Experimental group displayed significantly higher level of locomotion and anxiety, and showed lower sociability and ability to habituate to the repeated high-frequency sound. Learning capacity and cognitive rigidity did not differ between the groups. These results show that the treatment with 5HTP has influenced 5HT-regulated bahaviours, and indicate changes in the central 5HT compartment that occurred during brain development.

• Keywords: 5- hidroksitriptofan; serotonin; štakor; ponašanje; hiperserotoninemija
• Keywords (english): 5- hydroxytryptophan; serotonin; rat; behaviour; hyperserotonemia
• Language: croatian
• URN:NBN: urn:nbn:hr:217:745937
• Study programme: Title: Biology; specializations in: Ecology; Course: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-04-04 09:13:13