Imunohistokemijsko značenje biljega ciklina-D1 i p27 u procjeni metastatskog potencijala papilarnog mikrokarcinoma štitne žlijezde

master's thesis

• Title (english): Immunohistochemical significance of cyclin D1 and p27 in evaluation of metastatic potential of thyroid papillary microcarcinoma
• Author: Natalija Biškup
• Mentor: Fabijan Knežević (mentor)
• Mentor: Vesna Benković (sumentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Papilarni mikrokarcinom štitne žlijezde definiran je kao papilarni karcinom koji je manji ili jednak jednom centimetru. Papilarni mikrokarcinom ima odličnu prognozu dok se agresivnije ponašanje tumora očituje pojavom proširenja bolesti u limfne čvorove vrata. Poremećaj kontrole stanične diobe osnova je kancerogeneze. Stanični ciklus kontroliraju ciklin ovisne kinaze koje se aktiviraju formirajući komplekse s ciklinima. Kod većih papilarnim karcinoma pojava metastaza povezana je sa smanjenom izraženosti p27 i pojačanom aktivnost ciklina D1. Izraženost ciklina D1 i p27 odredili smo imunohistokemijskom analizom. Intraglandularna diseminacija u drugi režanj štitnjače je značajan pokazatelj agresivnog ponašanja tumora i pojave metastaza u limfne čvorove vrata (p<0,001). Veličina tumora je u negativnoj korelaciji s izraženošću p27 u jezgri (P=0,036). Izraženost ciklina D1 u jezgri pokazala je statistički značajnu negativnu korelaciju s izraženošću p27 u jezgri (P=0,023) i s izraženošću p27 u citoplazmi (P=0,001). Kod papilarnog mikrokarcinoma izraženost ciklina D1 i p27 nije u statistički značajnoj korelaciji s pojavom metastaza.

Abstract (english)

Thyroid papillary microcarcinoma is a papillary carcinoma less than or equal to one centimeter in diameter. Papillary mikrocarcinoma has an excelent prognosis if has no metastasis in regional lymph nodes. Alteration in control of the cell division is a basis of carcinogenesis. Cell cycle is controlled by cyclin-dependent kinases activated through complexes with cyclins. Studies on papillary carcinomas showed that underexpression of p27 and overexpression of cyclin D1 correlate with metastases. We determined expression of cyclin D1 and p27 using immunohistochemical assay. Appearance of intraglandular dissemination in another lobe of the thyroid is a significant factor of tumor aggressive behavior and appearance of metastases in regional lymph nodes (p<0.001). Size of a tumor negatively correlate with nuclear expression of p27 (P=0.036). Nuclear expression of cyclin D1 shows statistically significant negative correlation with nuclear and cytoplasmic expression of p27 (P=0.023 and P=0.001, retrospectively). Expression of cyclin D1 and p27 did not significantly correlate with appearance of metastases in thyroid papillary microcarcinoma.

• Keywords: cikoin D1; p27; karcinom štitnjače; prognostički čimbenik; imunohistokemija
• Keywords (english): cyclin-D1; p27; thyroid cancer; prognostic factor; immunohistochemistry
• Language: croatian
• URN:NBN: urn:nbn:hr:217:297094
• Study programme: Title: Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra edukacije biologije (magistar/magistra edukacije biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-04-12 11:24:02