Polimorfizmi ABC transportera i njihova uloga u tumorskim stanicama

undergraduate thesis

• Title (english): Polymorphisms of ABC transporters and their role in cancer cells
• Author: Lana Grižančić
• Mentor: Petra Korać (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

ABC transporteri su membranski proteini koji omogućuju prijenos različitih tvari kroz membranu i kao takvi su nužni u održavanju integriteta i homeostaze cjelokuponog metabolizma. Ti proteini se sastoje od više domena, a upravo je interakcija među domenama i njihova konformacijska promjena ključna u procesu prijenosa tvari, a kao glavni pokretač je energija dobivena hidrolizom ATP-a koja uzrokuje tu konformacijsku promjenu. S obzirom na homologiju domena, geni ABC porodice se mogu svrstati u 7 potporodica koje obnašaju različite funkcije u organizmu i zastupljene su u različitim stanicama i tkivima organizma. Različite vrste ABC transportera su odgovorne za prijenos supstrata koji mogu biti heterogene strukture, podrijetla i funkcije u organizmu. Iako sudjeluju u prijenosu endogeno sintetiziranih supstrata kao i stranih tvari, prvenstveno su otkriveni na temelju uloge u uklanjanju protutumorskih lijekova iz tumorskih stanica zbog čega smanjuju efikasnost liječenja tumora. Na efikasnost liječenja tumora utječu, osim lijekova i neki drugi čimbenici organizma kao što su i polimorfizmi ABC transportera. Različite genske varijante za određeni protein utječu na njegovu pojavnost i mogućnost obavljanja funkcije u organizmu. Tako je na temelju istraživanja različitih malignih bolesti u ljudi pokazano da različite genske varijante ABC transportera utječu na ishode liječenja bilo da povećavaju ili smanjuju mogućnosti ozdravljenja. Daljnja istraživanja će doprinijeti razumijevanju polimorfizama u ABC transporterima i njihovu funkciju u prijenosu tvari kroz membranu te na taj način omogućiti kvalitetniji pristup u liječenju malignih bolesti.

Abstract (english)

ABC transporters are transmembrane proteins that enable translocation of various substances across cellular membranes, and as such are essential in maintaining integrity and homeostasis of whole metabolism. These proteins are composed of multiple domains, and interactions between domains and their conformational changes are crucial in translocation process. These transbembrane proteins utilize energy by hydrolysis of ATP, which causes a conformational change. ABC gene family are classified based on domain homology into seven subfamilies. Different ABC transporter proteins perform different functions in the body and are present in different cells and tissues of the body. Different types of ABC transporters are responsible for the transfer of substrates with heterogeneous structure, origin and function in the cell. Although they participate in the transfer of endogenously synthesized substrates and the foreign substances, they were primarily discovered because of their role in the removal of anticancer drugs from tumor cells thereby reducing the efficiency of tumor ttreatment. Efficiency of the treatment of tumors affects other than drugs, and other factors such as cell physiological condition and polymorphisms of ABC transporters. Different genetic variant coding for a particular protein affect its conformation and the possibility of maintaining its function in cells. Research based on various malignancies in humans has shown that different gene variants of ABC transporters affect outcomes - either increase or decrease treatment efficiency. Further research should contribute to understanding polymorphisms in ABC transporters and their function in translocation mechanisms across the membrane and thus allow better understanding and more efficient treatment of malignant diseases.

• Keywords: ABC transporteri; liječenje tumora
• Keywords (english): ABC transporters; tumor treatment
• Language: croatian
• URN:NBN: urn:nbn:hr:217:382969
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-04-26 09:30:11