Raznolikost virusa Cryphonectria hypovirus 1 na području Kašta

master's thesis

• Title (english): Diversity of Cryphonectria hypovirus 1 in Kašt area
• Author: Ivana Pavičić
• Mentor: Mirna Ćurković Perica (mentor)
• Mentor: Lucija Nuskern Karaica (pomoć pri izradi, neposredni voditelj)
• Committee member: Marin Ježić (član povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Committee member: Petar Kružić (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-02-23, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Gljiva mješinarka Cryphonectria parasitica uzročnik je raka kore pitomoga kestena (Castanea sativa). Virus Cryphonectria hypovirus 1 (CHV1) inficira gljivu C. parasitica te smanjuje njezinu virulenciju, fenomen koji nazivamo hipovirulencija te tako omogućava prirodnu biološku kontrolu raka kore pitomoga kestena. Vrsta CHV1 najbolje je do sada proučena vrsta hipovirusa i jedina prisutna u Europi, gdje postoje četiri podtipa ovog virusa. Prisutnost virusa dokazana je izolacijom virusne dsRNA, reverznom transkripcijom te amplifikacijom otvorenog okvira čitanja ORF A. Nakon sekvenciranja umnoženih fragmenata dobivene su djelomične sekvence ORF A duljine 1134 nukleotida, te su izrađena filogenijska stabla. Prevalencija virusa CHV1 na području Kašta iznosila je 50 %. Virusni sojevi s područja Kašta pripadaju istom, talijanskom podtipu, vrlo su srodni sojevima iz Slovenije, Italije i Švicarske, a manje srodni sojevima iz Španjolske, Njemačke i Francuske. Virusni sojevi s područja Kašta pripadaju genski vrlo srodnoj populaciji virusa, te se značajno ne razlikuju od starih sojeva izoliranih na području Hrvatske i Slovenije.

Abstract (english)

Sac fungus Cryphonectria parasitica is a causal agent of chestnut blight, a disease of sweet chestnut (Castanea sativa). Cryphonectria hypovirus 1 (CHV1) is a natural biocontrol agent of chestnut blight: it infects C. parasitica and reduces its virulence towards chestnut. This phenomenon is called hypovirulence. CHV1 is the best studied species of hypoviruses and the only one present in Europe, where four subtypes of this virus have been identified. CHV1 presence was determined by isolation of viral dsRNA, reverse transcription and amplification of open reading frame (ORF A). After sequencing of amplified fragments, partial ORF A sequences 1134 mucleotides long were obtained and phylogenetic trees constructed. The prevalence of CHV1 in Kašt was 50 %. All virus strains from Kašt are of the same, Italian subtype, and are closely related to strains from Slovenia, Italy and Switzerland, while they are more distantly related to strains from Spain, Germany and France. Virus strains from Kašt form genetically very similar population and they do not differ significantly from the old strains isolated in Croatia and Slovenia.

• Keywords: Cryphonectria parasitica; hipovirus; rak kore kestena; biološka kontrola; molekularna filogenija
• Keywords (english): Cryphonectria parasitica; hypovirus; chestnut blight; biological control; molecular phylogeny
• Language: croatian
• URN:NBN: urn:nbn:hr:217:476012
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-02-23
• Created on: 2018-05-04 10:56:14