Uzgoj viroida i fitoplazmi u kulturi izdanaka vrsta Gynura aurantiaca (Blume) DC. i Catharanthus roseus (L.) G. Don.

master's thesis

• Title (english): In vitro cultivation of viroid-infected shoots of Gynura aurantiaca (Blume) DC. and phytoplasmas-infected shoots of Catharanthus roseus (L.) G. Don
• Author: Iva Panjkota
• Mentor: Mirna Ćurković-Perica (mentor)
• Committee member: Dubravka Matković-Čalogović (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Citrus exocortis viroid (CEVd), Candidatus Phytoplasma asteris (soj HydB), Ca. Phytoplasma solani (soj SA-I), Ca. Phytoplasma ulmi (soj EY-C) i Ca. Phytoplasma pruni (soj KVI) biljni su patogeni koji uzrokuju bolesti različitih biljnih vrsta. U ovom istraživanju uzgajani su u uvjetima in vitro u biljkama domaćinima Gynura aurantiaca (Blume) DC. i Catharanthus roseus (L.) G. Don. U hranidbene podloge za uzgoj zaraženih biljaka dodani su biljni regulatori rasta, auksini i citokinini, s ciljem istraživanja kako njihov dodatak utječe na rast i simptome biljaka domaćina. Praćen je rast zaraženih i zdravih biljaka domaćina tijekom tri supkulture. Citokinini su u zdravim i viroidom zaraženim izdancima biljke G. aurantiaca uzrokovali slabiji rast, te su i nezaraženi i viroidom zaraženi izdanci bolje rasli na podlozi bez citokinina. Dodatak auksina indol-3-maslačne kiseline u hranidbenu podlogu uzrokovao je bolji rast izdanaka vrste C. roseus zaraženih različitim sojevima fitoplazmi, a u nekih je uzrokovao i povlačenje simptoma zaraze.

Abstract (english)

Citrus exocortis viroid (CEVd), ‘Candidatus Phytoplasma asteris' (strain HydB), ‘Ca. Phytoplasma solani' (strain SA-I), 'Ca. Phytoplasma ulmi' (strain EY-C) and 'Candidatus Phytoplasma pruni' (strain KVI) are plant pathogens that cause diseases of different plant species. In this study they were grown in vitro in host plants Gynura aurantiaca (Blume) DC. and Catharanthus roseus (L.) G. Don. Plant growth regulators, auxins and cytokinins, were supplemented to nutrient medium for cultivation of infected plants in order to test their effect on the growth and symptoms of host plants. The growth of infected and healthy host plants was monitored through three subcultures. In healthy and viroid-infected shoots of G. aurantiaca cytokinins suppresed growth; both non-infected and viroid-infected shoots showed better growth on the medium without cytokinins. Addition of auxin indole-3-butyric acid into medium caused better growth of C. roseus shoots infected with different phytoplasma strains and in some cases it caused remission of symptoms.

• Keywords: auksin; citokinin; Citrus exocortis viroid; Candidatus Phytoplasma asteris; Ca. Phytoplasma solani; Ca. Phytoplasma ulmi; Ca. Phytoplasma pruni
• Keywords (english): auxin; cytokinin; Citrus exocortis viroid; Candidatus Phytoplasma asteris; Ca. Phytoplasma solani; Ca. Phytoplasma ulmi; Ca. Phytoplasma pruni
• Language: croatian
• URN:NBN: urn:nbn:hr:217:221307
• Study programme: Title: Biology and Chemistry; Study programme type: university; Study level: graduate; Academic / professional title: profesor biologije i kemije (profesor biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-05-17 09:51:07