Antibakterijski učinak zeolita sa sadržajem bakra

master's thesis

• Title (english): Antibacterial effect of copper - loaded zeolite
• Author: Marija Vujica
• Mentor: Jasna Hrenović (mentor)
• Mentor: Tomislav Ivanković (pomoć pri izradi, neposredni voditelj)
• Committee member: Jasna Hrenović (član povjerenstva)
• Committee member: Hrvoj Vančik (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Teški metali zbog svoje toksičnosti, stabilnosti i sklonosti nakupljanja u živim organizmima, predstavljaju ozbiljni ekološki problem. Stoga ih je potrebno ukloniti iz otpadnih voda prije ispuštanja u prirodni prijemnik. Prirodni zeolitni tuf pokazao se kao pogodan materijal s velikim kapacitetom vezanja iona Cu2+ iz otpadnih voda. U radu je ispitana antibakterijska aktivnost zeolitnog tufa koji sadrži 2,60 % masenog udjela (eng. percentage by weight, wt.%) Cu2+ u odnosu na bakterije Escherichia coli i Staphylococcus aureus, nakon 1 h i 24 h kontakta s zeolitom u tri vodena medija: hranjivom mediju Luria Bertani (LB), sintetskoj otpadnoj vodi i realnoj otpadnoj vodi. Utvrđeno je da zeolitni tuf obogaćen bakrom pokazuje antibakterijsku aktivnost u odnosu na Gram - pozitivnu bakteriju Staphylococcus aureus i Gram - negativnu bakteriju Escherichia coli. Nakon 24 h kontakta dolazi do redukcije broja bakterija u sintetskoj (87,35 – 95,67%) i realnoj otpadnoj vodi (86,79 – 93,54%), dok u Luria Bertani (LB) mediju (5,54 – 10,69%) redukcija nije bila učinkovita. Ne dolazi do izlučivanja toksičnih Cu2+ iona u vodenu otopinu što zeolit s Cu2+ čini obećavajućim antibakterijskim materijalom za dezinfekciju otpadnih voda.

Abstract (english)

Heavy metals pollution is one of the most important environmental problems, due of their toxicity, stability, and tendency to accumulate in living organisms. Therefore, heavy metals should be removed from the wastewater before it can be discharged into a natural recipient. Natural zeolitized tuff was proved to be a suitable material with high capacity binding of Cu 2+ ions from wastewater. The antibacterial activity of natural zeolitized tuff containing 2.60 percentage by weight (wt.%) Cu2+ were tested against bacteria Escherichia coli and Staphylococcus aureus after 1 h and 24 h of contact with zeolit in three different media, namely: Luria Bertani (LB) medium, synthetic wastewater and real wastewater. The copper – loaded zeolitized tuff showed antibacterial activity against Gram - positive bacteria Staphylococcus aureus and Gram - negative bacteria Escherichia coli, after 24 h of contact. The reduction of bacterial numbers was efficient in synthetic water (87,35 – 95,67%) and real effluent water (86,79 – 93,54%), while in the Luria Bertani (LB) medium the reduction was not efficient (5,54 – 10,69%). Since there was not leaching of toxic Cu2 + ions in the aqueous solution, Cu - loaded zeolite can be regarded as promising antibacterial material for disinfection of wastewater.

• Keywords: Escherichia coli; Staphylococcus aureus; otpadna voda; bakar; teški metali; zeolit
• Keywords (english): Escherichia coli; Staphylococcus aureus; wastewater; copper; hevy metals; zeolite
• Language: croatian
• URN:NBN: urn:nbn:hr:217:635803
• Study programme: Title: Biology and Chemistry; Study programme type: university; Study level: graduate; Academic / professional title: profesor biologije i kemije (profesor biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-05-18 09:16:10