Pokazatelji oksidacijskog stresa u duhanu (Nicotiana tabacum L.) izloženom talijevom (I) acetatu

master's thesis

• Title (english): Indicators of oxidative stress in tobacco (Nicotiana tabacum L.) exposed to thallium (I) acetate
• Author: Tanja Prigorec
• Mentor: Branka Pevalek-Kozlina (mentor)
• Mentor: Marija Babić (pomoć pri izradi, neposredni voditelj)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Oksidacijski stres uzrokovan metalima u biljaka se javlja kao sekundarna reakcija. Kao pokazatelje oksidacijskog stresa u izdancima duhana (Nicotiana tabacum L.) izloženim talijevom (I) acetatu pratila sam promjenu sadržaja proteina, vodikovog peroksida, malondialdehida i karbonila te promjenu aktivnosti enzima askorbat peroksidaze, pirogalol peroksidaze i katalaze. Biljke su tretirane s 2, 4, 20 i 40 μM Tl-acetata, 40 μM Tl-acetata i 40 μM K-acetata (kombinirani tretman), te 40 μM K-acetata koji je služio kao dodatna acetatna kontrola. Uzorci izdanaka duhana analizirani su nakon 3 dana. Sadržaj proteina, vodikovog peroksida i karbonila u odnosu na kontrolu se povećao dok se sadržaj malondialdehida nije značajno mijenjao. Aktivnost askorbat i pirogalol peroksidaze kao i aktivnost katalaze povećala se značajno u biljkama izloženim 2 μM i 4 μM Tl-acetata. Rezultati istraživanja pokazuju da talij izaziva oksidacijski stres u izdanku duhana, ali također ukazuju na učinkovitost antioksidacijskih mehanizama duhana pri različitim koncentracijama tog toksičnog metala upotrebljenim u ovom radu.

Abstract (english)

Oxidative stress in plants occurs as a secondary reaction to heavy-metals stress. In this work I analysed the change of protein content, hydrogen peroxide, malondialdehyde, carbonyls as well as activity of ascorbate peroxidase, pyrogallol peroxidase and catalase as indicators of oxidative stress in the shoots of tobacco (Nicotiana tabacum L.) exposed to thallium (I) acetate. Plants were treated with 2, 4, 20 and 40 μM Tl-acetate, 40 μM Tl-acetate and 40 μM K-acetate (combined treatment) and 40 μM K-acetate which served as an additional control of the acetate. Samples of tobacco shoots were analyzed after 3 days. The protein content, as well as hydrogen peroxide and carbonyl content, increased in relation to the control, while MDA content did not change. Ascorbate and pyrogallol peroxidase activity and catalase activity increased significantly in plants exposed to 2 μM and 4 μM Tl-acetate. The research results show that thallium causes oxidative stress in tobacco shoots, but also the efficiency of antioxidative mechanisms of tobacco at different concentrations of that toxic metal used in this work.

• Keywords: duhan; oksidacijski stres; talijev (I) acetat
• Keywords (english): tobacco; oxidative stress; thallium (I) acetate
• Language: croatian
• URN:NBN: urn:nbn:hr:217:209410
• Study programme: Title: Biology and Chemistry; Study programme type: university; Study level: graduate; Academic / professional title: profesor biologije i kemije (profesor biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-05-18 09:24:37