Dinamika nastanka diobenog vretena

master's thesis

• Title (english): Dynamics of mitotic spindle assembly
• Author: Barbara Kuzmić
• Mentor: Jasmina Rokov Plavec (mentor)
• Mentor: Iva Tolić (mentor)
• Committee member: Jasmina Rokov Plavec (predsjednik povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Josip Požar (član povjerenstva)
• Committee member: Marko Močibob (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2016-10-07, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Na početku diobe, stanica formira diobeno vreteno, molekularnu strukturu odgovornu za jednaku raspodjelu kromosoma među stanicama kćerima. To je vrlo kompleksna struktura koju čine mikrotubuli i pripadajući proteini. Tijekom prometafaze, kinetohorni mikrotubuli rastu iz polova diobenog vretena prema drugom kraju stanice i povezuju se s kinetohorama, multi-proteinskim strukturama na centromerama kromosoma. U ovom radu, fluorescencijska mikroskopija je korištena za vizualizaciju ljudskih stanica. Vizualizirane su i analizirane U2OS (CENP-A-GFP, mCherry--alpha-tubulin) stanice. Pokazano je da se duljina diobenog vretena smanjuje, a širina povećava tijekom prometafaze. Unatoč promjenama duljine i širine diobenog vretena, kontura ove stanične strukture ostaje nepromijenjena.

Abstract (english)

At the onset of division, the cell forms a spindle, a molecular machine responsible for equal distribution of chromosomes between the daughter cells. It is a highly complex structure made of microtubules and associated proteins. During prometaphase, kinetochore microtubules extend from poles on either end of the cell and attach to the kinetochores, multiprotein structures on the centromeres of the chromosomes. In this thesis, fluorescence microscopy was used for live-cell imaging of human cells. U2OS (CENP-A-GFP, mCherry-- alpha tubulin) cells were visualized and analyzed. It was shown that the spindle length decreases and the width increases during the prometaphase. Despite the changes in spindle length and width, the contour of this cell structure remains unchanged.

• Keywords: mitoza; prometafaza; diobeno vreteno; mikrotubuli; kinetohore; U2OS stanice; fluorescencijska mikroskopija
• Keywords (english): mitosis; prometaphase; mitotic spindle; microtubules; kinetochores; U2OS cells; fluorescence microscopy
• Language: croatian
• URN:NBN: urn:nbn:hr:217:249389
• Study programme: Title: Graduate Studies in Chemistry; specializations in: Research (scientific-professional) programme; Course: Research (scientific-professional) programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-01-26 12:39:05