Genska raznolikost gujavica rodova Aporrectodea Orley, 1885 i Lumbricus Linnaeus, 1758 (Oligochaeta, Annelida) u kontinentalnoj Hrvatskoj

master's thesis

• Title (english): Genetic variability in earthworms of genera Aporrectodea Örley, 1885 and Lumbricus Linnaeus, 1758 (Oligochaeta, Annelida) in continental Croatia
• Author: Lucija Hodak
• Mentor: Davorka Hackenberger Kutuzović (mentor)
• Mentor: Goran Klobučar (mentor)
• Committee member: Goran Klobučar (predsjednik povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-07-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology; Zoology

Abstract

Fauna gujavica u Hrvatskoj vrlo je raznolika i bogata vrstama, no još uvijek nedovoljno istražena. Mnoga dosadašnja istraživanja pokazala su da je određivanje vrste gujavica (posebice juvenilnih) na temelju morfoloških karakteristika vrlo složeno te da unutar opisanih vrsta vrlo vjerojatno postoji velik broj linija, odnosno podvrsta. Istraživanje je provedeno u svrhu utvrđivanja raznolikosti vrsta unutar rodova Aporrectodea i Lumbricus na temelju analize gena COI regije mtDNA. Na devet lokaliteta kontinentalne Hrvatske (Zagreb, Samoborsko gorje, Sunger, Kalnik, Ivanščica, Lipik, Orahovica, Osijek i Halaševo)morfološkom determinacijom izdvojeno je 33 jedinki roda Aporrectodea i 26 jedinki roda Lumbricus. Utvrđeno je ukupno 18 haplotipova za rod Aporrectodea i 16 haplotipova za rod Lumbricus te su zajedno sa pripadajućim sekvencama za te rodove preuzetima iz BOLD baze podataka (za područje Europe) konstruirani filogenetski odnosi. Molekularno-filogenetičke analize pokazale su da u Hrvatskoj unutar kompleksa vrsta A. caliginosa nije zabilježena vrsta A. caliginosa sensu stricto te da postoji visoka genska divergencija unutar vrsta A. rosea, L. terrestris i L. rubellus što može upućivati na nove linije ili vrste. Utvrđeno je preklapanje pojedinih haplotipova roda Lumbricus iz Hrvatske i Skandinavije što se može povezati s migracijama nakon posljednjeg ledenog doba. Da bi se dobila kompletna slika raznolikosti faune gujavica u Hrvatskoj, potrebno je provesti istraživanja na većem broju uzoraka.

Abstract (english)

The earthworm fauna of Croatia is very diverse and rich in species, but still insufficiently explored. Studies have shown that the determination of earthworm species (especially juvenile) on the basis of morphological characteristics is very complex and that large number of lineages, i.e. subspecies can be found within the established species. The study was conducted to determine the diversity of species within the genera Aporrectodea and
Lumbricus using analysis of mtDNA COI gene sequences. Using morphological analysis of collected specimens from nine localities of continental Croatia (Zagreb, Samoborsko gorje, Sunger, Kalnik, Ivanščica, Lipik, Orahovica, Osijek and Halaševo) 33 Aporrectodea and 26 Lumbricus specimens were chosen for molecular analyses. A total of 18 haplotypes were identified within the Aporrectodea genus and 16 haplotypes within Lumbricus genus, and phylogenetic relationships were constructed with the sequences from the BOLD database (Europe). Molecular phylogenetic analyses showed that there was no A. caliginosa specimens recorded within specimens belonging to A. caliginosa complex, while high genetic divergence within the established species A. rosea, L. terrestris and L. rubellus was revealed which may indicate new lineages or species of earthworms. It was found that some haplotypes of
Lumbricus genus from Croatia and Scandinavia overlap, what can be related to migrations after the last ice age. In order to obtain a complete picture of the variety of earthworm fauna in Croatia, it is necessary to conduct research on larger number of samples.

• Keywords: rod Aporrectodea; rod Lumbricus; filogenetika; kompleks vrsta; COI
• Keywords (english): genus Aporrectodea; genus Lumbricus; phylogenetics; species complex; COI
• Language: croatian
• URN:NBN: urn:nbn:hr:217:043561
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-08-29
• Created on: 2018-08-29 08:08:05