Karakterizacija adhesoma u stanicama karcinoma pločastog epitela jezika Cal27 nakon de novo ekspresije integrina αvß3

master's thesis

• Title (english): Characterisation of adhesome in tongue squamous carcinoma cells Cal27 upon de novo expression of integrin αvß3
• Author: Kristina Čuljak
• Mentor: Andreja Ambriović Ristov (mentor)
• Committee member: Morana Dulić (predsjednik povjerenstva)
• Committee member: Željka Soldin (član povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Biserka Prugovečki (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2018-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Vezanjem na izvanstanični matriks, integrini regrutiraju raznovrsne proteine oko
citoplazmatskog repa stvarajući strukture poznate kao fokalne adhezije. Skup svih molekula
povezanih s adhezijom integrina naziva se adhesom integrina. U ovom smo radu
okarakterizirali adhesome stanica pločastog epitela jezika Cal27 i klona Cal27-2B1,
dobivenog transfekcijom stanica Cal27 plazmidom koji sadrži cDNA za podjedinicu integrina
β3 i za kojeg je pokazana otpornost na nekoliko protutumorskih lijekova i povećana
pokretljivost. Izolirani su kompleksi adhezije integrina stanica Cal27 i Cal27-2B1 i analizirani
pomoću spektrometrije masa. Otkriveno je 639 različitih proteina od kojih je 19 dio
konsenzus adhesoma. U Cal27-2B1 otkriveno je 17 proteina koji se nalaze u većim
količinama nego u stanicama Cal27. Ovaj rad pruža vrijedan izvor informacija o sastavu
adhesoma karcinoma pločastog epitela jezika i promjenama u signalnim putevima integrina
koje su potencijalno uključene u fenotip povećane otpornosti na protutumorske lijekove i
povećanu pokretljivost čime bi se mogle otkriti nove mogućnosti za terapiju tumora.

Abstract (english)

Upon ligation to the extracellular matrix, integrins recruit various proteins to form structures
known as focal adhesions. The entire collective of molecules associated with integrin
adhesions was termed the integrin adhesome. In the present study we characterised adhesomes
of tongue squamous carcinoma cells Cal27 and Cal27-derived clone Cal27-2B1, obtained by
transfection of plasmid containing integrin subunit β3 cDNA and showing resistance to
several antitumor drugs, increased migration and invasion. The integrin adehesion complexes
from Cal27 and Cal27-2B1 were isolated and analysed using mass spectrometry identifying
639 different proteins with 19 out of 60 consensus adhesome proteins. In clone Cal27-2B1, 17
proteins were detected at much higher levels compared with Cal27 cells providing a valuable
resource of information that might reveal novel opportunities for therapeutic intervention in
tumors.

• Keywords: adhesom; fokalne adhezije; integrin αvβ3; karcinom pločastog epitela jezika
• Keywords (english): adhesom; focal adhesion; integrin αvβ3; tongue squamos carcinoma cells Cal27
• Language: croatian
• URN:NBN: urn:nbn:hr:217:212111
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-09-26
• Created on: 2018-11-19 13:00:48