Razvoj metode spektrometrije masa temeljene na podatkovno neovisnoj akviziciji za identifikaciju serumskih biomarkera lišmanioze u pasa

master's thesis

• Title (english): Development of mass spectrometry method based on data independent acquisition for identification of serum biomarkers in canine leishmaniosis
• Author: Marin Popović
• Mentor: Anita Horvatić (mentor)
• Committee member: Aleksandra Maršavelski (predsjednik povjerenstva)
• Committee member: Davor Kovačević (član povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Morana Dulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2018-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Lišmanioze su u mnogim krajevima svijeta postale zaboravljene tropske bolesti, no ponajviše
klimatske promjene uzrokuju širenje bolesti na nova područja gdje do sada nisu bile
zabilježene. Uzročnici ovih bolesti su različite vrste bičaša iz roda Leishmania, a prenose ih
različite vrste sitnih insekata, koji se u Hrvatskoj zovu papatači. Težina bolesti varira od
subkliničkih do vrlo teških oblika, a vrlo rijetko invazije mogu završiti i smrtno. Psi su
rezervoari uzročnika, a ljudi slučajne žrtve. Zbog toga je važno je pravovremeno
dijagnosticirati bolest i liječiti invadirane životinje.
Imajući na umu navedeno, kvantitativni proteomski pristup visoke rezolucije
primijenjen je za profiliranje proteoma seruma pasa. Razvijena je metoda spektrometrije masa
temeljena na podatkovno neovisnoj akviziciji (DIA) te testirana na kliničkim arhivskim
uzorcimaseruma kontrolne skupine (zdravih) i bolesnih pasa (kojima je serološkim metodama
dijagnosticirana lišmanioza). Nova metoda DIA je brza, jednostavna i osjetljivija te
omogućuje identifikaciju potencijalnih biomarkera lišmanioze u pasa.

Abstract (english)

Leishmaniasis is one of the most dangerous neglected tropical diseases which has (re)-
emerged in many geographic regions, mostly due to climatic changes. It is caused by
protozoan parasites of the genus Leishmania, transmitted by the bite of a female phlebotomine
sandfly. The severity of disease may vary from non-symptomatic to fatal. Infected dogs are
the main reservoir for human infection. Due to its zoonotic potential, it is very important to
diagnose the disease, and treat infected animals.
Whithin this aim, the high-resolution quantitative proteomic approach has been
employed for canine serum proteome profiling. The mass spectrometry (MS) method based
on data independent acquisition (DIA) was developed and tested using clinical samples -
serum of healthy and Leishmania-infected dogs. The new DIA MS method is simple, fast and
more sensitive, and provides a detailed insight into canine serum proteome, enabling the
identification of potential biomarkers for canine leismaniasis

• Keywords: biomarkeri; lišmanioza pasa; podatkovno neovisna akvizicija; proteomika; spektrometrija masa
• Keywords (english): biomarkers; canine leishmaniasis; data independent aquisition; mass spectrometry; proteomics
• Language: croatian
• URN:NBN: urn:nbn:hr:217:333060
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-09-26
• Created on: 2018-11-22 10:45:28