Adhezijska svojstva fitoplanktonske vrste Dunaliella tertiolecta Butcher u stresnim uvjetima

master's thesis

• Title (english): Adhesion behavior of phytoplankton specie Dunaliella tertiolecta Butcher under stress conditions in vitro
• Author: Nives Novosel
• Mentor: Nadica Ivošević DeNardis (mentor)
• Mentor: Marija Gligora Udovič (mentor)
• Committee member: Marija Gligora Udovič (predsjednik povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Vlasta Ćosović (član povjerenstva)
• Committee member: Neven Bočić (član povjerenstva)
• Committee member: Nenad Buzjak (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-11-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U ovom radu ispitan je utjecaj saliniteta i kadmija na brzinu rasta, fiziološki odgovor, oblik stanica i adhezijsko ponašanja na elektrodi, fitoplanktonske morske vrste Dunaliella tertiolecta Butcher. Korištene su mikroskopske i elektrokemijske metode polarografije i kronoamperometrije na kapajućoj živinoj elektrodi. D. tertiolecta nema čvrstu, celuloznu staničnu stijenku za razliku od ostalih zelenih algi, što je čini osjetljivom na promjene u ekosustavu i zbog čega je često korišten modelni organizam u istraživanjima stresa. Prilagodba stanica D. tertiolecta u uvjetima nižeg saliniteta u kulturi ukazuje na nešto veću dimenziju stanice, promjenu u sferični oblik stanice, te nešto brži rast, dok je adhezijsko svojstvo prianjanja u širokom području potencijala ostalo sačuvano. Prilagodba stanica na prisustvo teškog metala kadmija u kulturi rezultirala je smanjenjem gustoće stanica, smanjenjem dimenzije, promijenom oblika, pojavom površinski-aktivnih čestica i mikroagregata u mediju, što ukazuje na promijenjene metaboličke aktivnosti stanica uslijed stresa. Rezultati ovog rada doprinose boljem razumijevanju adhezijskog ponašanja stanica fitoplanktona u stresnim uvjetima in vitro, te unaprijeđuju znanje o preživljavanju i prilagodbi fitoplanktonskih organizama na prirodnim međupovršinama u vodenom okolišu u uvjetima opterećenja.

Abstract (english)

In this Thesis is examined the influence of salinity and presence of heavy metal cadmium in culture on the response of marine phytoplankton Dunaliella tertiolecta Butcher in terms of growth rate, physiological activity, cell shape and adhesion behavior on the electrode. Microscopic and electrochemical methods of polarography and chromoamperometry at the dropping mercury electrode were used. D.tertiolecta cell has no solid cellular cell wall in contrast to other green algae, which makes it susceptible to changes in the ecosystem and is why a model organism is often used in stress research. Adaptation of D. tertiolecta cell culture in lower salinity conditions suggests a slightly larger cell dimension, a change in spherical cell shape, a somewhat faster growth, while the adhesion properties at the broader potentials area remains preserved. Adaptation of phytoplankton to the presence of heavy metal cadmium in culture resulted in decrease of cell density with increasing cadmium concentration, cells are slightly smaller in size and show a deterioration in shape, the occurrence of surface active particles and microagregates in the culture which could be the result of the changed metabolic activity of cells due to stress. These results contribute to a better understanding of the adhesion behavior of phytoplankton cells in stress conditions in vitro and improve knowledge about survival and adaptation of phytoplankton organisms at natural interfaces in the aquatic environment under pressure.

• Keywords: adhezija; Dunaliella tertiolecta; fitoplankton; kadmij; salinitet; stres
• Keywords (english): adhesion; cadmium; Dunaliella tertiolecta; phytoplankton; salinity; stress
• Language: croatian
• URN:NBN: urn:nbn:hr:217:813648
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2019-11-13
• Created on: 2018-12-04 11:52:38