Identifikacija razgradnih produkata ceritiniba vezanim sustavom tekućinska kromatografija ultravisoke djelotvornosti - tandemna spektrometrija masa

master's thesis

• Title (english): Identification of degradation products of ceritinib by ultra high performance liquid chromatography - tandem mass spectrometry
• Author: Martina Kovačević
• Mentor: Nives Galić (mentor)
• Committee member: Nives Galić (predsjednik povjerenstva)
• Committee member: Željka Soldin (član povjerenstva)
• Committee member: Morana Dulić (član povjerenstva)
• Committee member: Snežana Miljanić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2019-02-21, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Ceritinib je farmaceutik koji se koristi u liječenju specifičnog oblika tumora pluća. Djeluje kao inhibitor kinaze anaplastičnog limfoma. Degradacijski produkti su neželjene tvari koje mogu nastati tijekom proizvodnje ili skladištenjem lijeka. Mogu utjecati na učinkovitost lijekova, stoga je vrlo važno istražiti stabilnost lijeka i odrediti njegove razgradne produkte.
U ovom diplomskom radu korištena je metoda tekućinske kromatografije ultravisoke djelotvornosti (UHPLC) za odjeljivanje ceritiniba i razgradnih produkata nastalih procesom forsirane razgradnje u kiselom, lužnatom i oksidirajućem mediju. Vezani sustav tekućinske kromatografije spregnute s tandemnom spektrometrijom masa (LC-MS/MS) korišten je pri analizi razgradnih produkata. Na temelju nastalih fragmenata pretpostavljene su strukture 7 razgradnih produkata ceritiniba i predloženi odgovarajući fragmentacijski putovi.

Abstract (english)

Ceritinib is a pharmaceutical used to treat a specific type of lung cancer. This molecule acts as an inhibitor of anaplastic lymphoma kinase. Degradation products are unwanted compounds that can be developed during the manufacturing and storaging of a drug. Development of these products can affect the efficacy of pharmaceuticals, therefore the examination of drug stability, and determination of it's degradation products is very important.
In this diploma thesis degradation products were generated through force degradation study in acidic, basic and oxidizing media. Degradation products and ceritinib were separated by high performance liquid chromatography (UHPLC). Degradation products were analysed by LC-MS/MS hyphenated system. Based on the resulting fragments, structures of 7

• Keywords: ceritinib; forsirana razgradnja; MS/MS; razgradni produkti; UHPLC metoda
• Keywords (english): ceritinib; degradation products; forced degradation; MS/MS; UHPLC method
• Language: croatian
• URN:NBN: urn:nbn:hr:217:607270
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-02-21
• Created on: 2019-03-13 17:47:32