Promjene sastava lipida diatomeje Chaetoceros pseudocurvisetus ovisno o dostupnosti nutrijenta fosfata

master's thesis

• Title (english): Lipid -composition changes of the diatom Chaetoceros pseudocurvisetus depending on the phosphate availability
• Author: Matea Bertanjoli
• Mentor: Blaženka Gašparović (mentor)
• Committee member: Iva Juranović Cindrić (predsjednik povjerenstva)
• Committee member: Aleksandra Maršavelski (član povjerenstva)
• Committee member: Željka Soldin (član povjerenstva)
• Committee member: Ines Primožič (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2019-02-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Sistematsko razumijevanje prilagodbe fitoplanktona, glavnog proizvođača lipida mora, na stres u smislu sinteze lipida još je u začetku. Istražene su promjene sastava lipida diatomeje Chaetoceros pseudocurvisetus u ovisnosti o dostupnosti fosfata. C. pseudocurvisetus pripremljen je na dva načina, jedan prekondicioniran u mediju bogatom fosfatom, a drugi u uvjetima manjka fosfata. C. pseudocurvisetus prekondicioniran u bogatom mediju presađen je u bogati medij i u medij osiromašen fosfatom. C. pseudocurvisetus prekondicioniran u fosfat osiromašenom mediju presađen je u medij osiromašen fosfatom. Ovim radom dobiven je uvid u promjenu sastava lipida u ovisnosti o dostupnosti fosfata, neophodnim za izgradnju fosfolipida stanične membrane, te je potvrđena mogućnost da C. pseudocurvisetus u uvjetima viška fosfata akumulira stanične rezerve fosfata koje koristi kada izvori fosfata izostanu. Lipidi su analizirani tankoslojnim kromatografom IATROSCAN MK-6 (TLC/FID-FPD).

Abstract (english)

The systematic understanding of phytoplankton, a major marine lipid producers, in regard to its adaptation to stress in terms of lipid synthesis is still in the early stages. Lipid-composition changes of the diatom Chaetoceros pseudocurvisetus, depending on the phosphate availability, were explored. C. pseudocurvisetus was prepared in two ways: one which was preconditioned in a phosphate-rich medium and the other in phosphate deficient conditions. C. pseudocurvisetus cells that were preconditioned in a phosphorus (P)-rich medium were inoculated into both the enriched and the P-depleted medium. C. pseudocurvisetus cells that were preconditioned in a P-depleted medium were inoculated into a P-deficient medium. This thesis provides insight into lipid-composition changes depending on the availability of P, which is necessary for the phospholipid synthesis, the fundamental building blocks of all cell membranes. It is also shown that C. pseudocurvisetus in conditions of phosphate excess can accumulate cellular P reserves, which can be used in a conditions of an absence of phosphate sources. A IATROSCAN MK-6 (TLC/FID-FPD) thin-layer chromatograph was used for lipid analyses.

• Keywords: alge; fitoplankton; fosfat; IATROSCAN; kromatografija; lipidi; nutrijenti
• Keywords (english): algae; chromatography; IATROSCAN; lipids; nutrients; phosphate; phytoplankton
• Language: croatian
• URN:NBN: urn:nbn:hr:217:001567
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-02-28
• Created on: 2019-03-20 17:04:12