Prenosivost virusa sjemenom u mahunarki

master's thesis

• Title (english): Seed transmition of viruses in legumes
• Author: Helena Justić
• Mentor: Dijana Škorić (mentor)
• Committee member: Dijana Škorić (predsjednik povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Committee member: Ivana Šola (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-02-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Virus mozaika krastavca, virus obiĉnog mozaika graha i virus mozaika repe znaĉajni su i poznati biljni patogeni sa širokim krugom domaćina koji ĉesto uzrokuju štetu na kultiviranim biljkama. Uz visoku infektivnost i patogenost, pojavi i širenju virusnih infekcija doprinose razliĉite mogućnosti horizontalnog prijenosa virusa. Postotak prijenosa navedenih virusa vertikalnim putem tj. sjemenom je malen, ali od izuzetnog znaĉaja u uvjetima monokulturnog uzgoja. Ĉlanovi nedavno otkrivene porodice Endornaviridae se prenose iskljuĉivo vertikalno; perzistiraju u domaćinskoj biljci i ne uzrokuju bolesti, a njihova prisutnost je fenotipski neprimjetna. Prenosivost sjemenom je zajedniĉka biološka znaĉajka ovih virusa koji djelomiĉno dijele i krug domaćina. Cilj rada je istraţiti prisutnost navedenih virusa u biljkama dobivenima iz komercijalno dostupnog sjemena razliĉitih vrsta mahunarki, kao i popratnu simptomatologiju, te potvrditi njihovu vertikalnu prenosivost i krug domaćina. Ovo je takoĊer prvo istraţivanje endornavirusa u Hrvatskoj. Rast i razvoj biljaka praćen je makroskopski. Iz listova razvijenih biljaka ekstrahirana je dvolanĉana RNA (dsRNA) te analizirana elektroforetski i spektrofotometrijski. Ekstrakti su podvrgnuti reverznoj transkripciji i lanĉanoj reakciji polimeraze (RT-PCR) s poĉetnicama razliĉite specifiĉnosti za detekciju ĉetiri virusa. Potencijalno virusni amplikoni su sekvencirani i analizirani. Na temelju rezultata još se ne moţe potvrditi prenosivost oĉekivanih virusa sjemenom odnosno prisutnost u obraĊenoj skupini uzoraka.

Abstract (english)

Cucumber mosaic virus, Bean common mosaic virus and Turnip mosaic virus are well-known and globally distributed plant pathogens that represent a threat to the crop production. The efficient horizontal transmission contributes to their high infectivity and virulence. Although vertical transmission via seed occurs rarely, it can adversely influence the plants grown in monoculture and increase the virus distribution. Members of a recently discovered family Endornaviridae are transmitted exclusively vertically and have a persistent lifestyle without causing apparent disease symptoms. Seed transmission is a biological feature shared among above mentioned viruses which also have partially overlapping host ranges. The purpose of this thesis is to test the presence of these viruses in the leguminous plants grown from commercially available seeds, as well as to investigate symptoms, host range and confirm seed-transmissibility. This was also the first attempt to detect endornaviruses in Croatia. Growth and development of the seedlings were monitored. Double-stranded RNA (dsRNA) was extracted from fully developed leaves and analyzed electrophoretically and spectrophotometrically. DsRNA samples were reverse-transcribed and amplified using primers targeting the above mentioned viruses. Amplicons were sequenced and analyzed. As yet, the research failed to confirm the presence of these viruses in the analyzed group of legume seedlings.

• Keywords: BCMV; CMV; dsRNA; Endornavirus; RT-PCR; TuMV
• Keywords (english): BCMV; CMV; dsRNA; Endornavirus; RT-PCR; TuMV
• Language: croatian
• URN:NBN: urn:nbn:hr:217:376991
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-20 09:48:34