Polimorfizimi gena TLR4 u tumorigenezi sporadičnog karcinoma debelog crijeva

master's thesis

• Title (english): TLR4 polymorphisms in sporadic colon cancer tumorigenesis
• Author: Anamarija Salar
• Mentor: Sanja Kapitanović (mentor)
• Mentor: Inga Marijanović (mentor)
• Committee member: Inga Marijanović (predsjednik povjerenstva)
• Committee member: Martina Šeruga Musić (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-02-21, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Sporadični karcinom debelog crijeva je jedan od najčešćih zloćudnih tumora u svijetu. Receptor TLR4 je ključni regulator koji pomaže u održavanju ravnoteže između mikrobioma i imunološkog sustava crijeva. Poremećena homeostaza uslijed promijenjene ekspresije TLR4 bi mogla biti ključna značajka u patogenezi karcinoma debelog crijeva. Poznata su dva polimorfizma gena TLR4 +896A/G i +1196C/T za koje je pokazana povezanost s nastankom tumora. Cilj ovog diplomskog rada je ispitati postoji li povezanost polimorfizama +896A/G i +1196C/T sa sklonošću za obolijevanje od sporadičnog karcinoma debelog crijeva te postoji li povezanost s preživljenjem oboljelih. Rezultati nisu pokazali povezanost ispitivanih polimorfizama TLR4 s povećanim rizikom obolijevanja od sporadičnog karcinoma debelog crijeva niti s preživljenjem oboljelih. Učestalost varijantnih alela +896 G i +1196 T bila je statistički značajno veća kod zdravih ispitanika u odnosu na učestalost kod ispitanika oboljelih od sporadičnog karcinoma debelog crijeva. Homozigotni nosioci varijantnog alela +896 G i +1196 T bili su statistički značajno zastupljeniji u populaciji zdravih ispitanika. Genotipovi polimorfizama +896A/G i +1196C/T gena TLR4 su bili vezani na ispitivanoj populaciji te su se nasljeđivali u tri kombinacije genotipova (AA,CC; AG,CT i GG,TT).

Abstract (english)

Sporadic colon cancer is one of the most common malignant tumors in the world. TLR4 receptor is a key regulator that helps maintain balance between the microbial and immune system in the gut. Abnormal homeostasis in altered TLR4 expression could be a key feature in colon cancer pathogenesis. TLR4 polymorphisms +896A/G and +196C/T are known to be associated with tumorigenesis. The aim of this graduate thesis is to examine whether there is a correlation between polymorphisms +896A/G and +1196C/T and sporadic colon cancer susceptibility as well as with the patient's survival. Our results showed no correlation of TLR4 polymorphisms with sporadic colon cancer susceptibility or with survival. The incidence of variant alleles +896 G and +1196 T was significantly higher in healthy controls compared to patient's with sporadic colon cancer. Homozygous carriers of variant alleles +896 G and +1196 T were statistically significantly more present in the population of healthy controls. TLR4 polymorphisms +896A/G and +1196C/T cosegregate in our sample, and are inherited in three combinations of genotypes (AA,CC; AG,CT i GG,TT).

• Keywords: TLR4; polimorfizmi gena; sporadični karcinom debelog crijeva
• Keywords (english): TLR4; polymorphisms; sporadic colon cancer
• Language: croatian
• URN:NBN: urn:nbn:hr:217:036627
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-02-21
• Created on: 2019-03-26 10:03:24