Molekularna karakterizacija bakterijskih zajednica tala Skradinskog buka

master's thesis

• Title (english): Molecular characterization of bacterial communities in different soils from Krka National park
• Author: Adriana Martinović
• Mentor: Sandi Orlić (mentor)
• Mentor: Silvija Černi (mentor)
• Committee member: Silvija Černi (predsjednik povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Committee member: Jasmina Rokov Plavec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-12-14, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Bakterije predstavljaju brojnu i široko rasprostranjenu skupinu mikroorganizama s važnom ulogom u evoluciji, no još su nedovoljno istražena skupina organizama. S ciljem istraživanja uloge bakterija u ekosustavu tla nužno je poznavati njihovu taksonomiju te populacijsku strukturu. Cilj ovog rada bio je molekularno karakterizirati bakterijske zajednice u uzorcima tla prikupljenim na lokalitetu Skradinski buk u Nacionalnom parku Krka metodama sekvenciranja sljedeće generacije te utvrditi eventualni utjecaj vegetacije na sastav analiziranih bakterijskih zajednica. Hipoteza istraživanja bila je da različiti uzorci tla imaju različiti sastav bakterijskih zajednica te da prisutnost vegetacije uvjetuje prisutnost određenih bakterijskih skupina u ispitivanom uzorku. Analizom dobiveni slijedovi nukleotida ukazuju na veliku raznolikost bakterijskih zajednica što je u skladu s činjenicom da se radi o području koje je pod visokim stupnjem zaštite te su ljudske djelatnosti u njemu ograničene. Pojedine porodice bakterija javljaju se u svim uzorcima u podjednakim udjelima kao što su porodice Verrucomicrobiaceae, kao najzastupljenija, te Sphingomonadaceae, Bryobacteraceae i Zoogloeceae koje su zastupljene u manjem udjelu. Veća zastupljenost porodice Verrucomicrobiaceae uočena je u tlima bogatim vegetacijom što se može tumačiti kao posljedica međusobne interakcije bakterijskih vrsta ove porodice i biljne vegetacije kao eukariotskih organizama

Abstract (english)

Bacteria represent a numerous and widespread group of organisms with a important role in evolution. Despite the important role they are still insufficiently researched groups of organisms. For determining the role of bacteria in soil ecosystems, their taxonomy and biodiversity should be known. The aim of this paper is the molecular characterization of bacterial communities in soil samples collected at the Skradinski buk placed in Krka National Park using the sequencing method of the next generations and determine the possible influence of vegetation on the composition of bacterial communities in analyzed soil samples. The hypothesis is that different soil samples show a large diversity of bacterial communities and that the presence of vegetation will cause the presence of certain bacterial families in the tesedt soil. The results of this study are presented in nucleotide sequences which show that different soil samples are characterized by many different bacterial communities. Some families of bacteria appear in all samples in equal shares such as family Verrucomicrobiaceae, as most present in each sample, and Sphingomonadaceae, Bryobacteracea and Zoogloeceae in a smaller proportion. Greater representation of family Verrucomicrobiaceae was shown in soils rich in vegetation which can be interpreted as a result of reciprocal links that bacterial species and plant vegetation and the fact that these bacteria present in soils rich in vegetation such as grassland because of the interconnectedness with eucariotic organisms.

• Keywords: ekosustav; bakterijske populacije; tlo; sedra; sekvenciranje
• Keywords (english): ecosystem; bacterial population; soil; tuff; sequencing
• Language: croatian
• URN:NBN: urn:nbn:hr:217:379784
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-12-14
• Created on: 2019-03-28 11:12:47