Utjecaj organskog opterećenja na sastav i brojnost bakterijskih populacija u morskom sedimentu ribogojilišta zadarskoga arhipelaga

master's thesis

• Title (english): Influence of organic load on bacterial population structure in marine sediment at fish farm in the Zadar archipelago
• Author: Sandro Vuk
• Mentor: Sandi Orlić (mentor)
• Mentor: Silvija Černi (mentor)
• Committee member: Vesna Petrović Peroković (predsjednik povjerenstva)
• Committee member: Nenad Judaš (član povjerenstva)
• Committee member: Silvija Černi (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-12-10, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Bakterije morskih ekosustava široko su rasprostranjene i slabo istražene, iako predstavljaju brojnu i značajnu mikrobnu skupinu. Da bi se utvrdila uloga bakterija u biogeokemijskim procesima nužno je poznavanje njihove taksonomije te vremenske i prostorne rasprostranjenosti. Cilj rada bio je usporediti sastav bakterijskih populacija u morskom sedimentu ispod ribogojilišta, te na lokalitetima udaljenima 50 m i 100 m od ribogojilišta. Metoda odabrana za taksonomsku karakterizaciju bakterijskih populacija bilo je sekvenciranje sljedeće generacije temeljeno na analizi gena za 16S rRNA. Pretpostavka istraživanja bila je da je zbog suviška hrane te metaboličkih produkata riba organsko opterećenje u sedimentu ispod ribogojilišta veće što može imati utjecaj na strukturu bakterijskih populacija. Rezultati su potvrdili da je bioraznolikost bakterija u sedimentu ispod samog ribogojilišta veća od one zabilježene na udaljenijim lokalitetima. Najveći broj identificiranih bakterija pripada upravo skupinama s važnom ulogom u preradi organskih molekula.

Abstract (english)

Marine ecosystems bacteria are widespread and poorly researched although they represent a very large and significant microbial group. In order to determine the role of bacteria in biogeochemical processes, it is necessary to know their taxonomy as well as time and spatial distribution. The aim of this paper was to compare the composition of bacterial populations in the marine sediment below the fish farm, and the localities 50 m and 100 m away from the fish farm. The assumption of the study is that due to excessive food and metabolic fish products the organic load in the sediment below the fish farm is higher. The method used for taxonomic characterization of bacterial populations is the next-generation sequencing method based on 16S rRNA gene analysis. The results indicate that the biodegradability of bacteria in the marine sediment below the fish farm itself is greater than that observed in the distant localities. The largest number of bacteria identified belongs to groups important in the processing of organic molecules, which may indicate the selection effect of organic load within the fish farm

• Keywords: ekosustav; populacija; taksonomija; sekvenciranje; bioraznolikost; 16S rRNA
• Keywords (english): ecosystem; population; taksonomy; sequencing; fish farm; biodiverisity; 16S rRNA
• Language: croatian
• URN:NBN: urn:nbn:hr:217:455994
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-12-12
• Created on: 2019-03-28 11:26:05