Genotipizacija izolata virusa hepatitisa A iz epidemije u Hrvatskoj 2017.

master's thesis

• Title (english): Molecular typing of hepatitis A virus isolates from epidemic in 2017. in Croatia
• Author: Josip Ujević
• Mentor: Ivana Grgić (mentor)
• Mentor: Silvija Černi (mentor)
• Committee member: Silvija Černi (predsjednik povjerenstva)
• Committee member: Zlatko Liber (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-02-18, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Virus hepatitisa A (HAV) uzročnik je hepatitisa A, akutnog oboljenja jetre. Virus se prenosi fekalno-oralnim putem, direktnim kontaktom sa zaraženom osobom ili konzumacijom zaražene hrane. Analizom genomske regije VP1-2A razlikuje se šest HAV-genotipova. Genotipovi koji inficiraju čovjeka su I, II i III s podtipovima A i B. Različiti genotipovi pokazuju različitu geografsku distribuciju. Prema podacima Europskog centra za sprječavanje i kontrolu bolesti u 2017. broj oboljelih od hepatitisa A u Europi neočekivano je porastao. U Hrvatskoj je krajem iste godine zabilježen porast broja zaraženih, prvo unutar populacije muškaraca koji imaju spolne odnose s muškarcima (MSM), a zatim i u općoj populaciji. Sekvenciranjem dijela VP1/P2A regije Sangerovom metodom te analizom sekvencija identificirano je ukupno sedam sojeva, koji pripadaju genotipu IA i IB. Iz rezultata istraživanja može se zaključiti da je u Hrvatskoj bilo ukupno sedam nezavisnih izvora zaraze. Dva soja virusa UK/Spain i Europride, koja su povezana s europskom epidemijom, dominiraju u zaraženih osoba u Hrvatskoj te su pretežito prisutna u MSM-populaciji. U općoj populaciji je identificirano pet sojeva virusa, za koji način prijenosa nije utvrđen. Dobiveni podaci o genotipovima i podtipovima analiziranih izolata mogu poslužiti za molekularno epidemiološku studiju rekonstrukcije puta rasprostranjivanja virusa hepatitis A unutar i između zaraženih populacija.

Abstract (english)

Hepatitis A virus (HAV) causes hepatitis A, an acute liver disease. It is transmitted by fecal-oral route, by direct contact with infected person or by contaminated food consumption. Six genotypes are distinguished by analyzing the genomic region VP1-2A. Genotypes, I, II and III, divided into subtypes A and B, infect humans. Different genotypes show different geographical distribution. According to the European Center for Disease Prevention and Control data, the number of hepatitis A cases in Europe unexpectedly increased in 2017. By the end of the same year, there was an increase in the number of infected in Croatia, first noticed in population of men who have sex with the men (MSM) and then in general population. Seven virus outbreak strains were detected by Sanger sequencing of VP1/P2A region followed by sequence analysis. Results suggest seven independent sources of infection in Croatia. Two virus strains, UK/Spain and EuroPride, linked to epidemic in Europe, dominate in Croatia and are predominantly present in MSM population. In general population, five strains of virus have been detected, for which method of transmission has not been elucidated. The observed results can be used for molecular epidemiological reconstruction of the virus transmission between and within infected population.

• Keywords: virusni hepatitis; VP1-2A regija; genotipovi; sekvenciranje; molekularna filogenija
• Keywords (english): viral hepatitis; VP1-2A region; genotype; sequencing; molecular phylogenetics
• Language: croatian
• URN:NBN: urn:nbn:hr:217:940941
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-04-02 08:55:38