Raznolikost gena DRB1 skupine II glavnog sustava tkivne podudarnosti u divljih svinja (Sus scrofa) s područja Medvednice

master's thesis

• Title (english): Variability of major histocompatibility complex class II DRB1 gene in wild boars (Sus scrofa) from Medvednica
• Author: Maja Balažin
• Mentor: Ana Galov (mentor)
• Committee member: Ana Galov (predsjednik povjerenstva)
• Committee member: Gordana Lacković-Venturin (član povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Renata Matoničkin Kepčija (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015-10-20, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Glavni sustav tkivne podudarnosti (engl. major histocompatibility complex, MHC) ključan je u pokretanju obrambenih mehanizama kralježnjaka, a smatra se da su neki od MHC lokusa među najpolimorfnijim u kralježnjaka. Raznolikost MHC gena utječe na sposobnost populacije da se obrani od različitih patogena. Glavni sustav tkivne podudarnosti kod svinja
naziva se SLA (engl. swine leukocyte antigen). Divlja svinja (Sus scrofa) je jedna od najprilagođenijih vrsta kralježnjaka, te kao domadar mnogim parazitima pogodna je vrsta za istraživanje korelacije između MHC haplotipa i podložnosti bolestima. Cilj istraživanja bio je otkriti imunogenetičku raznolikost u populacija divlje svinje s područja Medvednice analizom lokusa DRB1. U istraživanom uzorku od 49 jedinki, pronađeno je sedam alela, od čega je jedan novi alel. Prema omjeru nesinonimnih i sinonimnih supstitucija potvrdili smo da na DRB1 lokus ne djeluje pozitivna selekcija. Dodatnom analizom djelovanja selekcije na
pojedinačnim kodonima, utvrdili smo da je jedino kodon 48 pod utjecajem pozitivne selekcije. Rezultati ovog istraživanja će poslužiti za daljna istraživanja korelacija između MHC haplotipova i specifičnih parazitskih bolesti divljih svinja, što će u konačnici pridonijeti razumijevanju koevolucije domaćina i patogena i održavanja genetičke raznolikosti u populacijama životinja.

Abstract (english)

Major histocompatibility complex (MHC) plays a major role in initiating defense mechanisms of vertebrates. It is believed that some of the MHC loci are the most polymorphic in vertebrates. The variability of MHC genes affects population`s ability to defend itself from various pathogens. Major histocompatibility complex (MHC) of pigs is known as swine leukocyte antigen (SLA). Wild boar is considered one of the most widespread species. As host of many parasites it is suitable species for testing correlation between MHC haplotypes and susceptibility to diseases. The goal of the research was to discover imunogenetic diversity in the population of wild boars from Medvednica by analysis of DRB1 loci. In the examined sample of 49 individuals, seven alleles were found, one of them was new. According to the ratio of nonsynonymous and synonymous substitutions we have confirmed that the DRB1 locus isn`t under positive selection. An additional analysis of the effects of selection on individual codons showed us that only codon 48 is under the effect of positive selection. Results of this study will be used in further study of correlation between the MHC haplotypes and specific parasitic diseases of wild boars that will contribute to understanding of coevolution of the host and pathogen and maintenance of genetic diversity in animal populations.

• Keywords: divlje svinje; MHC; genetička raznolikost; parazitske bolesti
• Keywords (english): Suidae; MHC; genetic variability; parasitic diseases
• Language: croatian
• URN:NBN: urn:nbn:hr:217:614981
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-21 08:45:56