Interakcije proteina p53 s proteinima obitelji p53, NME i GLI

master's thesis

• Title (english): Interactons between p53 and p53, NME and GLI protein family members
• Author: Ivan Bradić
• Mentor: Maja Herak Bosnar (mentor)
• Committee member: Jasmina Rokov Plavec (predsjednik povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Ivica Đilović (član povjerenstva)
• Committee member: Ita Gruić Sovulj (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2019-09-18, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Protein p53 transkripcijski je faktor koji regulacijom ekspresije mnogo različitih gena sudjeluje u supresiji tumora. Mutacije gena p53 uočene su u više od 50% tumora. Pokazano je da se mutirani protein p53 ponaša kao onkogen. U melanomu rijetko dolazi do mutacije gena p53, no uočeno je kako je tumor supresorska funkcija proteina p53 svejedno narušena. Pokazano je da određeni proteini obitelji p53, NME i GLI sudjeluju u razvoju melanoma. Naša je pretpostavka da mehanizmu inaktivacije divljeg tipa proteina p53 u melanomima pridonose određene proteinske interakcije. Zbog toga smo proveli ispitivanje mogućih interakcija između proteina p53 i proteina obitelji p53, NME i GLI. Dobiveni rezultati pokazali su da protein p53 stupa u interakciju s izoformama del133p53alfa, TAp73alfa, Tap73beta i delNp73alfa. Ovi rezultati dobiveni na egzogenim proteinima mogu ukazati na to koje se interakcije mogu očekivati na endogenim proteinima i koji bi proteini mogli poslužiti kao moguće mete u liječenju melanoma

Abstract (english)

p53 protein is a transcription factor that suppresses tumour formation through regulation of expression of many different genes. p53 gene is mutated in more than 50% of all tumours. It has been shown that mutations transform its tumour suppressor nature to an oncogenic one. p53 is rarely mutated in melanoma, however, his tumour suppressor activity in these tumours is still impaired. It has been shown that specific members of p53, NME and GLI protein family take part in melanoma formation. Therefore, we hypothesised that the p53 activity in melanoma could be impaired by interactions with certain proteins. Based on that, we have tested if p53 interacts with p53, NME and GLI protein family members. The obtained results show that p53 interacts with del133p53alfa, TAp73alfa, Tap73beta and delNp73alfa isoforms. These results, obtained with exogenous proteins, might be an indication of possible interactions with endogenous proteins and point out possible targets in treating melanoma.

• Keywords: melanom; proteinske interakcije; p53; p73; NME; GLI
• Keywords (english): melanoma; protein interactions; p53; p73; NME; GLI
• Language: croatian
• URN:NBN: urn:nbn:hr:217:130987
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-09-18
• Created on: 2019-09-27 11:29:35