Diferencijalna analiza proteoma staničnih linija karcinoma pločastih stanica jezika Cal27 i Cal27-2B1

master's thesis

• Title (english): Differential proteome analysis of of Cal27 and Cal27-2H1 human tonque
• Author: Ivan Faraho
• Mentor: Mario Cindrić (mentor)
• Mentor: Andreja Ambriović Ristov (mentor)
• Committee member: Biljana Balen (predsjednik povjerenstva)
• Committee member: Gordana Lacković-Venturin (član povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Committee member: Kristian Vlahoviček (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015-02-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Integrin v3 je transmembranski heterodimerni receptor koji posreduje u komunikaciji stanica s izvanstaničnim matriksom. Ima važnu ulogu u adheziji, proliferaciji, migraciji i preživljenju stanica, kao i otpornosti na citostatike. U radu je korištena stanična linija karcinoma pločastih stanica jezika čovjeka Cal27, te integrin 3-stabilno transficirani klon Cal27-2B1, koji de novo eksprimira integrin v3 na površini, te pokazuje povećanu ekspresiju integrina v5, smanjenu migraciju i povećanu otpornost na citostatike. Kako bi se dobio uvid u stanične mehanizme odgovorne za nastale promjene, diferencijalno su analizirani proteomi navedenih staničnih linija i identificirani su proteini kojima se razlikuju ekspresije u ovisnosti o ekspresiji integrina v3.

Abstract (english)

v3 integrin is a transmembrane heterodimeric receptor that mediate cell to cell and cell to ECM communication. It has an important role in adhesion, proliferation, migration and cell survival, as well as drug resistance. Focus of this research were human tongue squamous cell carcinoma cell line Cal27 and integrin 3 stably transfected clone Cal27-2B1, with de novo expression of integrin v3, overexpression of integrin v5, decreased cell migration and increased drug resistance. Proteomes of these cell lines were differentialy analized to get an insight into mechanisms responsible for observed changes, and proteins with different expressions depending on integrin v3 expression were identified.

• Keywords: integrini; otpornost na citostatike; 2D-PAGE; spektrometrija masa; karcinom jezika
• Keywords (english): integrins; drug resistance; 2D-PAGE; mass spectrometry; tongue cancer
• Language: croatian
• URN:NBN: urn:nbn:hr:217:878567
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-21 09:55:54