Prisutnost virusa Cryphonectria hypovirus 1 u kalusima kestena

master's thesis

• Title (english): Presence of Cryphonectria hypovirus 1 in chestnut calli
• Author: Valentina Karin
• Mentor: Mirna Ćurković Perica (mentor)
• Committee member: Mirna Ćurković Perica (predsjednik povjerenstva)
• Committee member: Dunja Leljak-Levanić (član povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Committee member: Silvija Černi (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015-12-10, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cryphonectria hypovirus 1 (CHV1) je dvolančani (ds)RNA virus bez kapside koji inficira fitopatogenu gljivu Cryphonectria parasitica, uzročnika raka kore pitomoga kestena Castanea sativa. Ovaj virus smanjuje virulenciju i reproduktivni kapacitet gljive i jako je važan u biološkoj kontroli raka kestenove kore. Ukoliko se hipovirulentna gljiva zaražena CHV1 virusom unese u aktivni rak uzrokovan virulentnim sojem gljive C. parasitica, prestaje širenje raka te se formira kalus. Cilj ovog istraživanja bio je odrediti prevalenciju virusa CHV1 u kalusima kestena. Istraživanje je provedeno na 24 kalusa s područja Ozlja i Markuševca. Uočena je jako velika zastupljenost virulentnih sojeva gljive C. parasitica u kalusima kestena. Čak 29,16% kalusa u svom tkivu imalo je samo virulentne sojeve gljive, 45,84% imalo je kombinaciju virulentnih i hipovirulentnih sojeva gljive, dok ih je tek 25% imalo samo hipovirulentne sojeve gljive. Pretpostavlja se da se to moglo dogoditi zbog spontanog gubitka virusa iz hipovirulentnih sojeva gljive ili zbog gubitka cijele hipovirulentne gljive na čije se mjesto naselila nova virulentna gljiva koja bi ponovo mogla izazvati infekciju na kalusima. Da bi se ustanovio razlog gubitka hipovirulencije, potrebno je pratiti promjene u kalusu kroz određeni vremenski period.

Abstract (english)

Cryphonectria hypovirus 1 (CHV1) is a double-stranded (ds)RNA virus without a capsid that infects phytopathogenic fungus Cryphonectria parasitica, the causal agent of chestnut blight. This virus reduces virulence and reproductive capacities of the fungus and thus is very important in biological control of the chestnut blight. If a hypovirulent fungus carrying CHV1 is introduced into an active canker caused by virulent C. parasitica strain, canker expansion ceases and callus is formed. The aim of this research was to evaluate the prevalence of CHV1 in chestnut calli. A total of 24 calli from Ozalj and Markuševac were included in the research. Observed presence of virulent fungal strains in chestnut calli was high. As many as 29.16% of calli had only virulent fungal strains in their tissue, 45.84% of calli had the combination of virulent and hypovirulent fungal strains, and only 25% of calli had solely hypovirulent fungal strains in their tissue. It is assumed that this could have happened because of the spontaneous loss of virus from hypovirulent fungal strains or due to the loss of whole hypovirulent fungal strain and colonization of the callus by a new, virulent strain which could reinitiate the infection. In order to ascertain the reason for the loss of hypovirulence, it is necessary to monitor changes in the callus over a longer period of time.

• Keywords: Cryphonectria parasitica; rak kestenove kore; kalus; hipovirus
• Keywords (english): Cryphonectria parasitica; chestnut blight; callus; hypovirus
• Language: croatian
• URN:NBN: urn:nbn:hr:217:258196
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-21 11:05:12