Analiza crijevnog mikrobioma u štakorskom modelu Alzheimerove bolesti

master's thesis

• Title (english): Analysis of intestine microbiome of Alzheimer's disease rat model
• Author: Maja Ledinski
• Mentor: Nada Oršolić (mentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Biljana Balen (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Alzheimerova bolest (AB) je progresivna neurodegenerativna bolest i glavni uzrok kognitivnih poremećaja i poremećaja u ponašanju industrijaliziranog društva. Os mikrobiom-crijeva-mozak sastoji se od dvosmjernog komuniciranja između središnjeg i enteričkog živčanog sustava i povezuje emocionalne i kognitivne centre u mozgu s perifernom funkcijom crijeva. U ovom istraživanju je korišten štakorski model AB (n=10), koji je izazvan intraperitonealnim injiciranjem aluminijevog klorida (10 mg/kg štakora) i D-galaktoze (60 mg/kg štakora) tijekom 28 dana. Iz crijevnog sadržaja štakora analiziran je broj kolonija bakterija rodova Lactobacillus i Bifidobacter, enzimska aktivnost mikrobioma te je sekvencirana DNA. Podaci sekvenciranja su obrađeni metodom hijerarhijskog klasteriranja i metodom analize glavnih komponenti. Dobiveni rezultati pokazali su: a) smanjeni broj probiotičkih bakterija u modelu AB u odnosu na kontrolnu skupinu; b) povećanu enzimsku aktivnost β-galaktozidaze, β-glukozidaze i β-glukuronidaze u modelu AB u odnosu na kontrolnu skupinu c) da nije došlo do velike promjene u sastavu crijevnog mikrobioma, ali je došlo do značajnih promjena u 46 vrsta bakterija koje ukupno čine 11,61% crijevnog mikrobioma kontrolne skupine. Temeljem dobivenih rezultata može se zaključiti da su smanjeni udio Lactobacillus i Bifidobacter, povećana enzimska aktivnost β-galaktozidaze, β-glukozidaze i β-glukuronidaze i smanjenje biomase crijevnog mikrobioma povezani s upalnim procesom izazvanim kod štakorskog modela AB.

Abstract (english)

Alzheimer's disease (AD) is progressive neurodegenerative disease and leading cause of cognitive and behaviour impairments of industrialized society. Microbiome-gut-brain axis is bidirectional communication between central and enteric nervous system, thus connecting emotional and cognitive centers in brain with peripheral gut function. In this research rat model of AD (n=10) was induced by intraperitoneal injection of aluminium chloride (10 mg/kg rat) and D-galactose (60 mg/kg rat) during 28 days. Dilutions were made from rat's colon content and streaked on selective plates for isolation of Lactobacillus and Bifidobacter, activity of bacterial enzymes was analyzed and DNA isolated for sequencing. Sequencing data was analyzed by hierarchical clustering and principal component analysis. Results showed: a) reduced number of probiotic bacteria in AD model compared to control group; b) increased activity of β-galactosidase, β-glucosidase i β-glucuronidase in AD model compared to control group c) there was no great changes in composition of intestine microbiome, but 46 bacterial species, which make 11,61% of intestine microbiome of control group, significantly changed. Based on these results it can be concluded that reduced number of Lactobacillus and Bifidobacter, increased enzyme activity of β-galactosidase, β-glucosidase i β-glucuronidase and reduced intestine biomass are connected with inflammation induced in AD rat model.

• Keywords: Alzheimerova bolest; mikrobiom; probiotici; os mikrobiom-crijeva-mozak
• Keywords (english): Alzheimer's disease; microbiome; probiotics; microbiome-gut-brain axis
• Language: croatian
• URN:NBN: urn:nbn:hr:217:348567
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-10-11
• Created on: 2019-10-10 08:24:42