Sinergijski učinak meda i antibiotika na klinički značajne bakterije višestruko otporne na antibiotike

master's thesis

• Title (english): The synergistic effect of honey and antibiotics on the growth of several medically significant bacteria displaying multiple antibiotic resistance
• Author: Antonia Ivešić
• Mentor: Amir Ibrahimagić (mentor)
• Mentor: Silvija Černi (mentor)
• Committee member: Silvija Černi (predsjednik povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Romana Gračan (član povjerenstva)
• Committee member: Marin Ježić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U današnje vrijeme raste broj klinički značajnih bakterija koje pokazuju višestruku otpornost na antibiotike. Istraživanja ukazuju na antimikrobni učinak meda, temeljen na fizikalno-kemijskim svojstvima i kemijskom sastavu meda. Hipoteza rada je da med povećava osjetljivost na antibiotike kod bakterija kojima je antibiogramom potvrđena višestruka otpornost. Svrha istraživanja je potvrditi antibakterijsko djelovanje bagremovog, kaduljinog, lipovog i livadskog meda. Antibakterijski potencijal ovih medova testiran je na osam bakterija: Acinetobacter baumanni, Citrobacter freundii, Enterobacter sakazakii, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Providentia rettgeri i Pseudomonas aeruginosa. Nakon analize antibiograma, uzorci su tretirani različitim koncentracijama meda te je svakoj vrsti meda, metodom razrjeđenja, utvrđena minimalna inhibitorna i minimalna baktericidna koncentracija. Nakon tretmana medom, ponovljen je antibiogram, zbog usporedbe rezultata antibiograma prije i nakon djelovanja meda. Mjerenjem zona inhibicije, jako antimikrobno djelovanje pokazali su med bagrema i kadulje u sinergizmu s antibiotikom sulfometoksazol-trimetoprim, no statistički značajni rezultati mjerenja zona inhibicije prije i nakon tretmana ispitivanim vrstama meda, pokazali su se jedino rezultati kod bakterije P. mirabilis, a analizom je utvrđeno kako med kadulje i livade imaju najveći utjecaj na porast inhibicijskih zona antibiotika. Istraživanje ukazuje da med može postati antimikrobno sredstvo, međutim, nepredvidiva i nestandardizirana antibakterijska aktivnost meda otežava njegovu primjenu kao antimikrobnog agensa.

Abstract (english)

Today, there is a growing number of clinically relevant bacteria showing resistance toward multiple antibiotics. Research indicates the antimicrobial effect of honey, based on the physicochemical properties and chemical composition of honey. The working hypothesis is that honey increases antibiotic susceptibility in bacteria that have been confirmed to be resistant to multiple drugs by antibiogram. The purpose of the study is to confirm the antibacterial activity of acacia, sage, linden and meadow honeys. The antibacterial potential of these honey was tested for eight bacteria: Acinetobacter baumanni, Citrobacter freundii, Enterobacter sakazakii, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Providentia rettgeri and Pseudomonas aeruginosa. After antibiogram analysis, the samples were treated with different concentrations of honey and a minimum inhibitory and minimum bactericidal concentration were determined for each type of honey by the dilution method. After the honey treatment, the antibiogram was repeated to compare the results of antibiograms before and after treatment of bacteria with honeys. Measurement of the zones of inhibition showed strong antimicrobial activity of acacia and sage honeys, having sinergistic effect with sulfomethoxazole-trimethoprim antibiotic. Statistically significant changes in zones of inhibition before and after treatment were obtained only in P. mirabilis, but the analysis showed that sage and meadow honeys have been found to influence the increase of antibiotic inhibition zones. Research indicates that honey can become an antimicrobial agent, however, the unpredictable and non-standardized antibacterial activity of honey hinders its use as an antimicrobial agens.

• Keywords: anitmikrobna tvar; antibiotska otpornost; terapija
• Keywords (english): antibiotic resistance; antimicrobial agent; therapy
• Language: croatian
• URN:NBN: urn:nbn:hr:217:232879
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-09-30
• Created on: 2019-10-14 09:01:04