Utjecaj antidepresiva i amfetamina na fiziološke pokazatelje u invazivne strane vrste signalnog raka, Pacifastacus leniusculus (Dana, 1852)

master's thesis

• Title (english): Effects of antidepressants and amphetamine on physiological parameters of the invasive alien species signal crayfish, Pacifastacus leniusculus (Dana, 1852)
• Author: Jelena Krsnik
• Mentor: Sandra Hudina (mentor)
• Mentor: Sofia Ana Blažević (mentor)
• Committee member: Sandra Hudina (predsjednik povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Committee member: Jasenka Sremac (član povjerenstva)
• Committee member: Mladen Maradin (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Farmaceutici u slatkovodnim ekosustavima mogu utjecati na ponašanje i fiziologiju vodenih
organizama jer su dizajnirani da brzo djeluju u ljudskom tijelu i izlučuju se bez razgradnje, pa
u vodu dospijevaju farmakološki aktivni. U ovom radu istražila sam utjecaj farmaceutika
prisutnih u rijeci Savi (antidepresivi citalopram i nortriptilin i opojna droga amfetamin) na
signalnog raka, najuspješniju invazivnu stranu vrstu raka u Europi. Rakove sam izlagala
okolišno relevantnoj (niskoj) ili terapeutskoj (visokoj) koncentraciji citaloprama, nortriptilina
ili amfetamina tijekom 7 dana. S obzirom da odabrani farmaceutici utječu na metabolizam
serotonina koji kod rakova kontrolira otpuštanje hiperglikemijskog hormona, mjerila sam
razinu serotonina u cerebralnim ganglijima i razinu glukoze u hemolimfi te malondialdehid
kao marker oksidativnog stresa. Rezultate izmjerenih parametara izloženih jedinki usporedila
sam s kontrolnima koje nisu bile izložene spojevima. Nortriptilin u visokoj koncentraciji
povisio je razinu serotonina u cerebralnim ganglijima u odnosu na grupu izloženu niskoj
koncentraciji. Kod rakova izloženih niskoj koncentraciji nortriptilina zabilježeno je povišenje
razine glukoze u hemolimfi u odnosu na kontrolne grupe, a izlaganje amfetaminu u obje
koncentracije snizilo je razine glukoze u hemolimfi. Nisam zabilježila povišenje markera
oksidativnog stresa. U budućim istraživanjima potrebno je produljiti vrijeme izlaganja i
istražiti utjecaj na ostale hormonima regulirane fiziološke parametre (rast i razmnožavanje).

Abstract (english)

Pharmaceutical pollution can affect behavior and physiology of freshwater organisms since
pharmaceuticals act quickly in the human body, excrete without degrading and enter
freshwaters still pharmacologically active. This study explores whether pharmaceuticals
present in the Sava River (antidepressants Citalopram and Nortriptyline, illicit drug
Amphetamine) have an effect on the physiology of the most successful crustacean invader of
European freshwaters, the Signal crayfish. I exposed individuals to environmentally relevant
(low), or therapeutic (high) concentration of Citalopram, Nortriptyline or Amphetamine
dissolved in ambient water for 7 days. Serotonin mediates the release of crustacean
hyperglycemic hormone (cHH) that controls hemolymph glucose concentration, and since
these chemicals alter serotogenic metabolism, I measured concentration of serotonin in the
brain, concentration of glucose in hemolymph, and malondialdehyde, marker of oxidative
stress, and compared the results of exposed individuals to that of control-unexposed groups.
High concentration of Nortriptyline elevated serotonin levels in comparison to exposure to
low concentrations. Glucose levels were higher than in control group at low concentration of
Nortriptyline, and lower than in control group at both concentrations of Amphetamine.
There was no influence on levels of oxidative stress biomarker. Future research should
include long-term exposure and examine effects of pharmaceuticals on growth and
reproduction.

• Keywords: farmaceutici; lipidna peroksidacija; hiperglikemija; serotonin
• Keywords (english): pharmaceuticals; lipid peroxidation; hyperglycemia; serotonin
• Language: croatian
• URN:NBN: urn:nbn:hr:217:948848
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-09-26
• Created on: 2019-10-14 09:29:43