Utvrđivanje povezanosti primarnih oštećenja DNA spermija s reproduktivnom sposobnošću muškaraca

master's thesis

• Title (english): Determining the relationship of primary DNA damages of sperm and fertile ability of men
• Author: Ante Ševo
• Mentor: Vesna Benković (mentor)
• Mentor: Patrik Stanić (mentor)
• Committee member: Vesna Benković (predsjednik povjerenstva)
• Committee member: Gordana Lacković-Venturin (član povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Committee member: Duje Lisičić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Humani spermij je muška reproduktivna haploidna stanica (23 kromosoma), koja se spaja sa humanom jajnom stanicom - ženskom reproduktivnom haploidnom stanicom (23 kromosoma) te zajedno stvaraju diploidnu stanicu, koja se razvija u novi organizam. Kvaliteta spermija, kao i nastanak spermija, ovise o genetskoj pozadini i promjenama, fizičkim traumama te mnogim egzogenim i endogenim čimbenicima u testisima i okolnom tkivu te sporednim organima koji čine dio reproduktivnog trakta. Analizom tradicionalnih sjemenih parametara prema WHO smjernicama te oštećenja DNA spermija komet testom utvrđena je međusobna povezanost navedenih parametara, kao i povezanost životnih navika i navedenih parametara. Oštećenje DNA spermija nije statistički značajno povezano s tradicionalnim sjemenim parametrima (koncentracija, pokretljivost i građa spermija te dob ispitanika). Konzumiranje cigareta i pripravaka biljke Cannabis ne utječe značajno na kvalitetu spermija, dok konzumiranje alkohola smanjuje pokretljivost i koncentraciju spermija.

Abstract (english)

Human sperm is male reproductive haploid cell (23 chromosomes) which merges with the human ovum - the female reproductive haploid cell (23 chromosomes), create a diploid cell, which develops into a new organism. The overall quality of the sperm, as well as the formation of the sperm, depends on the genetic background and changes, physical trauma, and many exogenous and endogenous factors in the testicles and surrounding tissue and secondary organs that form the reproductive tract. The analysis of traditional semen parameters according to WHO guidelines and DNA damage sperm by comet assay showed correlations of these parameters, as well as relationship between lifestyle and these parameters. Sperm DNA damage isn't significantly associated with traditional semen parameters (concentration, motility and sperm morphology and age of the examinee). The consumption of cigarettes and Cannabis does not significantly affect the quality of sperm, whereas alcohol consumption decreases the motility and sperm concentration.

• Keywords: humana stanica; testis; komet test; životne navike
• Keywords (english): human cell; testicle; comet assay; lifestyle
• Language: croatian
• URN:NBN: urn:nbn:hr:217:887642
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 13:04:32