Proteomska analiza promjena u stanicama tumora dojke čovjeka MDA-MB-435S nakon utišavanja integrina alfav

master's thesis

• Title (english): Proteomic analysis of human breast cancer cells MDA-MB-435S after silencing integrin alphav
• Author: Alen Kovačević
• Mentor: Mario Cindrić (mentor)
• Mentor: Andreja Ambriović Ristov (mentor)
• Committee member: Dubravko Pavoković (predsjednik povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Committee member: Antun Alegro (član povjerenstva)
• Committee member: Petra Peharec Štefanić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015-02-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Integrini su transmembranski glikoproteini koji stanici omogućuju kontakt s izvanstaničnim matriksom i drugim stanicama. Sudjeluju u prijenosu molekularnih signala čime reguliraju brojne procese u stanici kao što su adhezija, rast, preživljenje, diferencijacija i pokretljivost. Stanice tumora dojke čovjeka MDA-MB-435S eksprimiraju na svojoj površini heterodimere integrina αVβ3 i αVβ5. Utišavanjem ovih integrina, transfekcijom αV-specifičnom siRNA, stanice MDA-MB-435S postaju osjetljivije na djelovanje vinkristina i paklitaksela te im se smanjuje migratorna sposobnost. U ovom radu opisane su promjene u proteomskom profilu stanica MDAMB- 435S nakon utišavanja integrina αV. Ovo istraživanje moglo bi doprinjeti otkrivanju molekularnog mehanizma povećanja osjetljivosti stanica na vinkristin i paklitaksel i smanjenja migratorne sposobnosti, nakon utišavanja podjedinice integrina αV.

Abstract (english)

Integrins are transmembrane glycoproteins that mediate cell-cell and cell-ECM interactions. They are a part of signal transduction pathways that regulate numerous cell processes such as cell adhesion, growth, proliferation, differentiation and migration. Human breast carcinoma cell line MDA-MB-435S expresses integrins αVβ3 and αVβ5. The silencing of integrins αvβ3 and αvβ5, achieved by αv-specific siRNA transfection, increases sensitivity of MDA-MB-435S cells to vincristine and paclitaxel and reduces their migratory potential. In this study we describe changes in MDA-MB-435S proteome profile upon integrin αV silencing. These results could contribute to better understanding of molecular mechanisms involved in MDA-MB- 435S chemosensitation as well as mechanisms involved in decreased cell migration
after integin αV silencing.

• Keywords: tumor dojke; MDA-MB-435S; protutumorski lijekovi; integrini; proteomika
• Keywords (english): breast cancer; MDA-MB-435S; anti-cancer drugs; integrins; proteomics
• Language: croatian
• URN:NBN: urn:nbn:hr:217:708717
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-25 08:11:57