Određivanje bioraznolikosti faune tulara (Insecta, Trichoptera) u Hrvatskoj metodom barkodiranja DNA

doctoral thesis

• Title (english): Determination of caddisflies (Insecta, Trichoptera) fauna biodiversity in Croatia using the method of DNA barcoding
• Author: Anđela Ćukušić
• Mentor: Mladen Kučinić (mentor)
• Mentor: Martina Podnar Lešić (komentor)
• Committee member: Ana Previšić (predsjednik povjerenstva)
• Committee member: Branka Bruvo Mađarić (član povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-08-30, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

Tulari se zbog povezanosti sa specifičnim uvjetima staništa i osjetljivosti na onečišćenje, smatraju
ključnom bioindikatorskom skupinom na temelju koje su se brojne zemlje obavezale pratiti biološku
kakvoću slatkovodnih ekosustava. Pouzdano i brzo određivanje do razine vrste pokazalo se izazovnom
u taksonomiji. Fauna tulara u Hrvatskoj nepotpuno je istražena zbog malog broja specijaliziranih
taksonoma i nedostatka morfološkog ključa za određivanje pojedinih svojti ženki i ličinki. U ovom
radu korištena je metoda DNA barkodiranja, koja se pokazala kao brz i pouzdan alat za određivanje
nepoznatih uzoraka svrstavanjem u poznatu vrstu ili skupinu srodnih vrsta kroz filogenetsku analizu.
Istraženi su filogenetski odnosi 441 primjerka tulara prikupljenih na 177 lokacija u Hrvatskoj.
Uspješno je DNA barkodirano 85% poznatih vrsta tulara, odnosno 89% poznatih rodova u fauni
Hrvatske iz svih 17 porodica, a DNA barkodirajuće sekvence pohranjene su u svjetsku javnu bazu
BOLD. Metodom DNA barkodiranja potvrđeni su nalazi novih i rijetkih vrsta u fauni Hrvatske,
određeni primjerci koje nije bilo moguće odrediti korištenjem morfoloških značajki ili za koje nisu
opisane dijagnostičke značajke. Istaknuti su genetski divergentni primjerci unutar poznatih vrsta.
Otkriveno je šest do sada nezabilježene vrsta za znanost, uključujući i pet kriptičnih vrsta.

Abstract (english)

Caddisflies are used as biological indicators of water quality because they are highly sensitive to
environmental stress and pollution. Therefore, they have an essential role in standardized ecological
assessment of aquatic ecosystems in most countries. However, reliable and rapid identification to the
species level is proved challenging. In Croatia, caddisfly faunistic data are incomplete due to a small
number of taxonomists and a lack of morphological description keys for females and larvae of known
species. In this research, a DNA barcoding method was used as a fast and reliable tool that can classify
an unknown sample into a known species or group of related species through phylogenetic analysis.
Phylogenetic relations between 441 caddisfly individuals, sampled from 177 locations in Croatia are
analyzed in this study. A full-length DNA barcode was obtained from 85% of known caddisfly
species, representing 89% of known genera and all 17 families of caddisflies known from Croatia.
DNA barcode sequences are stored in the BOLD public database. The DNA barcoding method
confirmed findings of new and rare species in Croatian caddisfly fauna, identified specimens that were
not possible using morphology or for which no diagnostic features were described. Specimens with
highly divergent DNA barcode with respect to known species are highlighted. DNA barcoding
revealed six new species, including five cryptic species hitherto unrecorded based on morphology

• Keywords: tulari; COI; taksonomija; filogenija; vodeni kukci
• Keywords (english): caddisfly; COI; taxonomy; phylogeny; aquatic insects
• Language: croatian
• URN:NBN: urn:nbn:hr:217:045262
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-08-30
• Created on: 2020-02-05 09:43:09