Animalni model psorijaze: antiupalna i antioksidativna učinkovitost epigalokatehin galata

master's thesis

• Title (english): Animal model of psoriasis: anti inflammatory and antioxidant epigallocatechin gallate efficiency
• Author: Vedran Balta
• Mentor: Nada Oršolić (mentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Zora Popović (član povjerenstva)
• Committee member: Zdravko Dolenec (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-07-02, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Psorijaza je kronična upalna bolest, čije imunopatogeneza, prevencija i liječenje još uvijek nisu u cijelosti razriješene. Istražili smo moguće korisno djelovanje pripravka epigalokatehin galata (EGCG) na animalnom modelu psorijaze, prouzročene iritantom di-n-propil disulfidom (PPD), tijekom 5 dana. Antiupalno i antioksidativno djelovanje EGCG-a pratili smo kroz više različitih metoda kao što su određivanje broja stanica u trbušnoj šupljini, funkcionalna aktivnost makrofaga u peritonealnoj tekućini, patohistološka analiza promjena na koži pokusnih životinja, hematološke i biokemijske parametre krvi te metoda za procjenu antioksidativne učinkovitosti (reducirani glutation, lipidna peroksidacija). Rezultati ukazuju da pripravak EGCG-a smanjuje ukupan broj upalnih stanica u peritonealnoj tekućini miševa. Sve uočene promjene hematoloških i biokemijskih pokazatelja kretale su se unutar normalnih vrijednosti, a rezultati određivanja citokina u plazmi pokazali su da je PPD sam ili združen s EGCG-om izazvao veću razinu upalnih citokina, posebice, čimbenika stimulacije kolonija granulocita (G-CSF), zatim interleukina-6 (IL-6). Patohistološka analiza uzoraka kože pokazala je da združena topička primjena iritanta s testiranim tvarima u svim skupinama ima upalnu reakciju znatno slabijeg intenziteta od PPD-a. Genotoksični učinak istraživanih pripravaka procijenili smo pomoću komet testa i mikronukleus testa. Rezultati komet testa i mikronukleus testa pokazuju da EGCG nije genotoksičan za stanice periferne krvi miševa. Temeljem rezultata zaključujemo da istraživani pripravak EGCG-a, pokazuje protuupalni učinak, te da bi njegova primjena mogla poslužiti u sprječavanju i liječenju upalnih promjena psorijaze, bez toksičnih posljedica.

Abstract (english)

Psoriasis is a chronic, immune-mediated disease, which immunopathogenesis, prevention and treatments are not completely resolved. We did some researches about positive effects of epigallocatehin gallate (EGCG) preparations on an animal model of psoriasis, in which the psoriasis was caused by di-n-propyl disulfide (PPD) irritant during 5 days. Anti-inflammatory and anti-oxidative activity of ECGC preparations were followed by various methods: valuation of cell numbers in abdominal cavity, functional activity of macrophages in peritoneal fluid, pathohistological analyses of transformations on experimental animals, hematological and biochemical parameters of blood and method of valuation of anti-oxidative efficiency (reduced glutathione, lipid peroxidation). The results indicate that EGCG preparations reduce number of inflammatory cells in peritoneal fluid of mice. All hematological and biochemical transformations indicate that it falls within the reference range and the results of cytokines' values in plasma indicate that PPD alone, or in aggregation with EGCG, caused a bigger level of anti-inflammatory cytokines, especially colony stimulating factore hormones (G-CSF) and interleukins-6 (IL-6). Pathohistological analysis of skin samples indicates that a topical use of the irritant with tested substances, in all groups, causes significantly lower inflammatory reaction than PPD itself. We estimated genotoxic effect of examined preparations with the comet test and the micronucleus test. The results of tests show no genotoxicity induced by ECGC preparations in peripheral blood cells of mice. We concluded that researched ECGC preparations showed anti-inflammatory effect. It could be used for suppression and treatment of inflammatory presents in psoriasis, without any toxical effects.

• Keywords: flavonoidi; psorijaza; termografija; oksidacijski stres; upala
• Keywords (english): flavonoids; psoriasis; thermography; oxidative stress; inflammation
• Language: croatian
• URN:NBN: urn:nbn:hr:217:994889
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-25 12:19:38