Paired arrangement of kinetochores together with microtubule pivoting and dynamics drive kinetochore capture in meiosis I

Scientific paper - Original scientific paper

• Author: Gheorghe Cojoc
• Author: Florescu Ana-Maria
• Author: Alexander Krull
• Author: Klemm Anna H.
• Author: Nenad Pavin; MBZ: 204800
• Author: Frank Jülicher
• Author: Iva Marija Tolić; MBZ: 260543
• Author's institution: University of Zagreb; Faculty of Science; (Department of Physics)
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Physics
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract (english)

Kinetochores are protein complexes on the chromosomes, whose function as linkers between spindle microtubules and chromosomes is crucial for proper cell division. The mechanisms that facilitate kinetochore capture by microtubules are still unclear. In the present study, we combine experiments and theory to explore the mechanisms of kinetochore capture at the onset of meiosis I in fission yeast. We show that kinetochores on homologous chromosomes move together, microtubules are dynamic and pivot around the spindle pole, and the average capture time is 3-4 minutes. Our theory describes paired kinetochores on homologous chromosomes as a single object, as well as angular movement of microtubules and their dynamics. For the experimentally measured parameters, the model reproduces the measured capture kinetics and shows that the paired configuration of kinetochores accelerates capture, whereas microtubule pivoting and dynamics have a smaller contribution. Kinetochore pairing may be a general feature that increases capture efficiency in meiotic cells.

• Keywords (english): kinetochore capture; microtubule pivoting; microtubule dynamics
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Scientific Reports
• Numbering: vol. 6, 25736
• p-ISSN: 2045-2322
• DOI: https://doi.org/10.1038/srep25736
• URN:NBN: urn:nbn:hr:217:824945
• Publication: 2016
• Document URL: https://www.nature.com/articles/srep25736
• Type of resource: Text
• Access conditions: Open access
• Public note: © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/. Received: 28 January 2016. Accepted: 21 April 2016. Published: 11 May 2016.
• Created on: 2020-02-23 14:37:35