Analiza uloge međustanične komunikacije u simbiozi Rhizobium-Leguminosae

master's thesis

• Author: Franka Busch
• Mentor: Jasna Hrenović (mentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Petar Kružić (član povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Renata Matoničkin Kepčija (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016-02-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U istraživanju je provedeno ispitivanje uloge međustanične komunikacije u simbiozi Rhizobium-Leguminosae u svrhu boljeg razumijevanja međustanične komunikacije u procesu fiksacije dušika. Metodama molekularnog inženjerstva konstruirani su mutanti cinR i traI gena koji su dio genoma bakterije soja Rhizobium leguminosarum bv. viciae UPM791. Geni cinR i traI su izabrani iz razloga što imaju kontrolu nad acil-homoserin lakton ovisnom sistemu međustanične komunikacije. Mutanti gena cinR i traI su uspješno dobiveni i podvrgnuti daljnjim istraživanjem utjecaja mutanata na interakciju s biljkama. Preliminarni rezultati dobiveni TLC analizom ukazuju da cinR mutant smanjeno proizvodi C14-HSL molekule kao i kratkolančane AHL molekule. Analize traI mutanta ukazuju na smanjenu proizvodnju kratkolančanih AHL molekula. Preliminarne rezultate potrebno je potvrditi nastavkom istraživanja radi procjenjivanja značaja mutacija.

Abstract (english)

In this study the role of quorum sensing communication in Rhizobium-Leguminosae symbiosis was examined in the process of nitrogen fixation. In order to understand better the quorum sensing communication with the methods of molecular engineering mutants in cinR and traI genes, which are the part of the bacterial strains genome of Rhizobium leguminosarum bv. viciae UPM791, were constructed. Genes cinR and traI are chosen because they control the AHL-dependent quorum sensing system. Mutants of genes cinI and traI are successfully obtained and further characterized at the laboratory for phenotypes in interactions with plants. Preliminary results obtained by TLC analysis showed that the cinR mutant is producing less C14-HSL signals, also showing a reduction on the level of short-chain AHLs. Analysis of traI gene showed no significant alteration on C14-HSL production but the level of short-chain AHLs is reduced. Preliminary results have to be confirmed in further analysis to assess better examination of the significance of mutations

• Keywords: bakterije; signalne molekule; homoserin lakton; acil-homoserin lakton; nodulacija
• Keywords (english): bacteria; signal molecules; homoserin lactone; aycl-homoserin lactone; nodulation
• Language: croatian
• URN:NBN: urn:nbn:hr:217:417404
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-25 12:53:01