prikaz prve stranice dokumenta Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes
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Scientific paper - Original scientific paper
Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes
Developmental cell, 43 (2017), 1; 11-23. https://doi.org/10.1016/j.devcel.2017.09.010
Vukušić, Kruno; Buđa, Renata; Bosilj, Agneza; Milas, Ana; Pavin, Nenad; Tolić, Iva Marija

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Vukušić, K., Buđa, R., Bosilj, A., Milas, A., Pavin, N. & Tolić, I. M. (2017). Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes. Developmental cell, 43. (1), 11-23. doi: 10.1016/j.devcel.2017.09.010

Vukušić, Kruno, et al. "Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes." Developmental cell, vol. 43, no. 1, 2017, pp. 11-23. https://doi.org/10.1016/j.devcel.2017.09.010

Vukušić, Kruno, Renata Buđa, Agneza Bosilj, Ana Milas, Nenad Pavin and Iva Marija Tolić. "Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes." Developmental cell 43, no. 1 (2017): 11-23. https://doi.org/10.1016/j.devcel.2017.09.010

Vukušić, K., et al. (2017) 'Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes', Developmental cell, 43(1), pp. 11-23. doi: 10.1016/j.devcel.2017.09.010

Vukušić K, Buđa R, Bosilj A, Milas A, Pavin N, Tolić IM. Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes. Developmental cell [Internet]. 2017 [cited 2023 March 31];43(1):11-23. doi: 10.1016/j.devcel.2017.09.010

K. Vukušić, R. Buđa, A. Bosilj, A. Milas, N. Pavin and I. M. Tolić, "Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes", Developmental cell, vol. 43, no. 1, pp. 11-23, 2017. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:217:599330. [Accessed: 31 March 2023]

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Metadata
Title (english)Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes
AuthorKruno Vukušić
AuthorRenata Buđa
AuthorAgneza Bosilj
MBZ: 349215
AuthorAna Milas
AuthorNenad Pavin
MBZ: 204800
AuthorIva Marija Tolić
MBZ: 260543
Author's institutionUniversity of Zagreb
Faculty of Science
(Department of Physics)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract (english)
During cell division, mitotic spindle microtubules segregate chromosomes by exerting forces on kinetochores. What forces drive chromosome segregation in anaphase remains a central question. The current model for anaphase in human cells includes shortening of kinetochore fibers and separation of spindle poles. Both processes require kinetochores to be linked with the poles. Here we show, by combining laser ablation, photoactivation, and theoretical modeling, that kinetochores can separate without any attachment to one spindle pole. This separation requires the bridging fiber, a microtubule bundle that connects sister kinetochore fibers. Bridging fiber microtubules in intact spindles slide apart with kinetochore fibers, indicating strong crosslinks between them. We conclude that sliding of microtubules within the bridging fibers drives pole separation and pushes kinetochore fibers poleward by the friction of passive crosslinks between these fibers. Thus, sliding within the bridging fiber works together with the shortening of kinetochore fibers to segregate chromosomes.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleDevelopmental cell
Numberingvol. 43, no. 1, pp. 11-23
p-ISSN1534-5807
DOIhttps://doi.org/10.1016/j.devcel.2017.09.010
URN:NBNurn:nbn:hr:217:599330
Publication2017
Document URLhttps://www.sciencedirect.com/science/article/pii/S1534580717307335
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Public note© 2017 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). This work was funded by the European Research Council (ERC, GA number 647077). Published 9 October 2017.
Created on2020-02-28 10:39:36