Genetička raznolikost i genska identifikacija slatkovodnih vrsta mahovnjaka Hrvatske (Bryozoa: Gymnolaemata, Phylactolaemata)

master's thesis

• Title (english): Genetic diversity and gene identification of freshwater bryozoans in Croatia (Bryozoa: Gymnolaemata, Phylactolaemata)
• Author: Nikola Koletić
• Mentor: Damjan Franjević (mentor)
• Committee member: Damjan Franjević (predsjednik povjerenstva)
• Committee member: Sven Jelaska (član povjerenstva)
• Committee member: Kristian Vlahoviček (član povjerenstva)
• Committee member: Antun Alegro (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014-02-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Mahovnjaci su sitni beskralješnjaci koji nastanjuju sve tipove vodenih ekosustava. To su male životinje koje nespolnim pupanjem tvore velike zadruge koje mogu dostići veličinu do nekoliko desetaka centimetara, dok pojedinačne jedinke unutar kolonije imaju veličinu od nekoliko milimetara. Svaka jedinka unutar kolonije funkcionira kao zasebni zooid te je genetički identična svakoj drugoj jedinci unutar iste kolonije. Većina slatkovodnih vrsta mahovnjaka pripada u razred Phylactolaemata, dok par vrsta koje podnose bočatu vodu pripadaju u razred Gymnolaemata. Cilj ovog istraživanja bio je izolirati i umnožiti jezgrine gene za 18S rRNA, 28S rRNA i ITS2 regiju te mitohondrijski COI gen iz svih vrsta slatkovodnih mahovnjaka sakupljenih na području Hrvatske. Sekvence su poslužile za određivanje molekularno-filogenetskih odnosa slatkovodnih mahovnjaka zabilježenih u Hrvatskoj u usporedbi s morskim mahovnjacima čije sekvence su preuzete iz baze GenBank. Određivanjem haplotipova i konstrukcijom filogenetskih stabala potvrđena je genska identifikacija vrsta morfološki determiniranih i nedeterminiranih uzoraka sakupljenih mahovnjaka čime je napravljen prilog poznavanju rasprostranjenosti slatkovodnih i bočatih skupina mahovnjaka u Hrvatskoj.

Abstract (english)

Bryozoans are tiny invertebrates that inhabit all types of aquatic ecosystems. They are small animals that form large cooperatives by asexual budding, which can reach the size of several tens of centimeters, while individual units within a colony have a size of several millimeters. Each individual within a colony functions as a separate zooid and is genetically identical to each other individual within the same colony. Most freshwater species of bryozoans belong to the class Phylactolaemata, while several species that tolerate brackish water belong to the class Gymnolaemata. The aim of this study was to isolate and multiply nuclear genes for 18S rRNA, 28S rRNA and ITS2 region as well as the mitochondrial COI gene from bryozoans collected in Croatia. The sequences were used to determine the molecular phylogenetic relationships of freshwater bryozoans recorded in Croatia with marine bryozoans, whose sequences were retrieved from the GenBank database. The genetic identification of the morphologically determined and undetermined samples of bryozoans collected in Croatia was verified by determining the haplotype structure and using phylogenetic trees, which contributed to the knowlege about distribution of freshwater and brackish groups of bryozoans in Croatia.

• Keywords: slatkovodni mahovnjaci; Phylactolaemata; Gymanolaemata; 18S rRNA; 28S rRNA; ITS2; COI
• Keywords (english): freshwater bryozoa; Phylactolaemata; Gymanolaemata; 18S rRNA; 28S rRNA; ITS2; COI
• Language: croatian
• URN:NBN: urn:nbn:hr:217:770724
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-26 09:57:02