Djelotvornost eravaciklina na enterobakterije otporne na karbapenem u uvjetima in vitro

master's thesis

• Title (english): In vitro efficacy of eravacycline on carbapenem-resistant
• Author: Ivana Jurić
• Mentor: Ivana Mareković (mentor)
• Mentor: Ivana Ivančić Baće (sumentor)
• Committee member: Ivana Ivančić Baće (predsjednik povjerenstva)
• Committee member: Mirta Tkalec (član povjerenstva)
• Committee member: Jasna Hrenović (član povjerenstva)
• Committee member: Sunčica Bosak (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-12-18, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Enterobakterije otporne na karbapeneme (eng. Carbapenem-Resistant Enterobacteriaceae, CRE) su razvile sposobnost stvaranja beta-laktamaza KPC, VIM, NDM i OXA-48 zbog sve veće uporabe karbapenema u kliničkoj praksi te predstavljaju veliku opasnost za ljudsko zdravlje. CRE su, osim na karbapeneme, otporne i na većinu ostalih antibiotika te stoga otežavaju liječenje hospitaliziranih bolesnika. Nastoje se razviti novi antimikrobni lijekovi poput eravaciklina koji je odobren za liječenje teških intraabdominalnih infekcija kod odraslih osoba. Cilj ovog istraživanja je bio odrediti osjetljivosti 80 sojeva CRE, izoliranih iz kliničkih uzoraka bolesnika, na eravaciklin te njegovu djelotvornosti in vitro na CRE s obzirom na tip karbapenemaze koju stvaraju. Metodama disk difuzije i E-testa potvrđeno je da je 67,5 % sojeva osjetljivo na eravaciklin gdje je Klebsiella oxytoca pokazala najveću otpornost od 60 %. S obzirom na vrstu karbapenemaze, sojevi koji produciraju OXA-48 pokazali su najveću osjetljivost na eravaciklin s ukupno 71,4 % osjetljivih sojeva. Po osjetljivosti slijede VIM sa 65,4 % te NDM s ukupno 61,1 % osjetljivih sojeva. Rezultati istraživanja pokazali su djelotvornost eravaciklina u uvjetima in vitro na CRE, ali se uspješna terapija eravaciklinom za infekcije uzrokovanih s CRE treba temeljiti na ispitivanju osjetljivosti fenotipskim metodama, molekularnim određivanjem vrste karbapenemeze, te težini infekcije i osobitostima svakog pacijenta.

Abstract (english)

Carbapenem-resistant Enterobacteriaceae (CRE) have developed a mechanism for the production of KPC, VIM, NDM, and OXA-48 beta-lactamases due to the increasing use of carbapenem in clinical practice and pose a major risk to human health. CREs, in addition to carbapenem, are resistant to most other antibiotics and therefore make it difficult to treat hospitalized patients. New therapies have been sought to be approved, like eravacycline for the treatment of severe intra-abdominal infections in adults. This study aimedto determinate the susceptibilities of 80 strains of CRE, isolated from clinical patient specimens, to eravacycline and its in vitro efficacy to CRE concerning the type of carbapenemase they produce. Disc diffusion and E-test methods confirmed that 67,5 % of CRE were sensitive to eravacyclin where K. oxytoca showed the highest resistance of 60 %. Regarding the type of carbapenemase,the OXA-48 strains showed the highest susceptibility to eravacycline with a total of 71,4 % of susceptible strains, followed by VIM with 65,4 % and NDM with a total of 61,1 % of the susceptible strains. Test results have shown in vitroefficacy of eravacyclineon CRE, but successful therapy with eravacycline for CRE infections should be based on susceptibility testing with phenotypic methods, molecular determination of the type of carbapenemase, severity of the infection, and the characteristics of each patient.

• Keywords: eravaciklin; enterobakterije otporne na karbapenem (CRE); beta-laktamaze; karbapenemaze; infekcije
• Keywords (english): eravacycline; carbapenem-resistant enterobacteria (CRE); beta-lactamases; carbapenemases; infections
• Language: croatian
• URN:NBN: urn:nbn:hr:217:809954
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-03-08
• Created on: 2020-06-09 10:48:44