Molekularna karakterizacija kukaša (Acanthocephala) iz crijeva slatkovodnih riba

master's thesis

• Title (english): Molecular characterisation of intestinal parasites (Acanthocephala) in gut of freshwater fishes
• Author: Izabela Mujakić
• Mentor: Irena Vardić Smrzlić (mentor)
• Mentor: Davor Zanella (mentor)
• Committee member: Davor Zanella (predsjednik povjerenstva)
• Committee member: Sandra Radić Brkanac (član povjerenstva)
• Committee member: Neven Bočić (član povjerenstva)
• Committee member: Jasenka Sremac (član povjerenstva)
• Committee member: Danijel Orešić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014-11-17, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Koljeno kukaša (Acanthocephala) čine crijevni nametnici koji parazitiraju uglavnom na ribama te imaju složeni životni ciklus koji uključuje međudomadare, pripadnike faune dna (Ostracoda, Malacostraca i druge). Kod težih invazija mogu uzrokovati bolesti riba – akantocefaloze, a zahvaljujući sposobnosti akumulacije teških metala u visokim koncentracijama imaju ulogu bioindikatora u vodenim ekosustavima. Glavni ciljevi istraživanja bili su: istražiti rasprostranjenost i determinirati kukaše u ribama iz rijeka Matice, Gacke, Like-Novčica i Une. Uzorkovanje je provedeno tijekom tri godine (2010., 2011., 2012.) Određeni su osnovni biometrijski parametri riba-domadara te osnovni kvantitativni parametri invadiranosti kukašima: učestalost, medijan intenziteta invadiranosti te minimalan i maksimalan broj nametnika. Determinacija kukaša izvršena je morfološkom analizom i određivanjem sljedova nukleotida molekularnih biljega (18S rRNA, ITS i COI) umnoženih u lančanoj reakciji polimerazom (PCR). Determinirano je sedam vrsta kukaša: Echinorhynchus salmonis i Neoechinorhynchus rutili u rijekama Gackoj i Matici, Acanthocephalus lucii i Acanthocephalus anguillae u rijeci Novčici, Pomphorhynchus laevis, Pomphorhynchus tereticollis i Dentitruncus truttae u rijeci Uni. Velika bioraznolikost kukaša u ovim rijekama vjerojatno je posljedica kvalitetne i čiste vode, neophodne za naseljavanje različitih vrsta rakova koji su kukašima međudomadari.

Abstract (english)

Phylum of spiny-headed worms (Acanthocephala) are constituted of the parasites that attack mainly fish and have a complex life cycle that includes intermediate hosts, members of bottom fauna (Ostracoda, Malacostraca and others). With high rates of infestation they can cause fish disease - acanthocephalosis, and thanks to their ability to accumulate heavy metals in high concentrations, they have a role as bioindicators in aquatic ecosystems. The main objectives of the research were: to investigate the distribution and determine spiny headed worms in fish from rivers: Matica, Gacka Like-Novčića and Una. Sampling was conducted over three years (2010, 2011, and 2012.) The basic biometric parameters of fish hosts and basic quantitative parameters of spiny-headed worms infestation were determined: frequency, median intensity of infestation and the minimum and maximum number of parasites. Determination of spiny headed worms was performed with morphological analysis and sequencing of molecular markers (18S rRNA, ITS and COI) amplified in polymerase chain reaction (PCR). Seven species of spiny-headed worms were determined: Echinorhynchus salmonis and Neoechinorhynchus rutili in the rivers Gacka and Matica, Acanthocephalus lucii and Acanthocephalus anguillae in river Novčica, Pomphorhynchus laevis, Pomphorhynchus tereticollis i Dentitruncus truttae in river Una. Great biodiversity of spiny headed worms in these rivers is likely the result of high quality and clean water, necessary for the settlement of different types of crustaceans that are intermediate hosts for spiny headed worms.

• Keywords: kukaši; morfologija; 18S rDNA; ITS; podjedinica I citokrom c oksidaze; međudomadari
• Keywords (english): acanthocephalans; morphology; 18S rDNA; ITS; cytochrome c oxidase subunit; intermediate hosts
• Language: croatian
• URN:NBN: urn:nbn:hr:217:155436
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-26 10:48:26