Genetička raznolikost i struktura populacija vrste Gymnocalycium monvillei (Lem.) Britton & Rose (Cactaceae) duž visinskog gradijenta u planinskom području pokrajine Córdoba (Argentina)

master's thesis

• Title (english): Genetic diversity and structure of populations of the species Gymnocalycium monvillei (Lem.) Britton & Rose (Cactaceae) along an altitudinal gradient in the Sierras of Córdoba (Argentina)
• Author: Dragan Rašić
• Mentor: Zlatko Liber (mentor)
• Committee member: Zlatko Liber (predsjednik povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-02-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U planinskim se područjima povećanjem nadmorske visine mijenjaju klimatski i ekološki uvjeti, a što se zrcali u promjenama u sastavu vegetacije. Budući da se može pretpostaviti kako će vrste sa širokom rasprostranjenošću biti pod manjim utjecajem klimatskih promjena, nego što je to slučaj s usko rasprostranjenim vrstama, proučavanje vrsta tolerantnih na promjenu nadmorske visine osobito je zanimljivo u svjetlu današnjih klimatskih promjena. Cilj je ovog diplomskog rada provesti preliminarna populacijsko-genetička istraživanja triju prirodnih populacija vrste G. monvillei (Cactaceae) s različitih nadmorskih visina upotrebom AFLP-biljega (eng. Amplified Fragment Lenght Polymorphism markers). Nakon usporedbe dobivenih rezultata s rezultatima već provedenih morfoloških i fizioloških istraživanja, definirat će se opsežnija AFLP istraživanja na većem broju prirodnih populacija. Uočena genetska raznolikost, vrijednosti DW-indeksa (eng. frequency down-weighted marker), rezultati analize PCoA (eng. Principal Coordinate Analysis) i STRUCTURE analize ukazuju na dugu vremensku izoliranost populacije s najviše nadmorske visine i slobodan protok gena kod dviju populacija na nižim nadmorskim visinama. Jedinke populacija s nadmorske visine od 1250 m, zbog utvrđene najveće genetičke raznolikosti, globularne građe klijanaca, otpornosti na povišenu temperaturu i sušu, velikih plodova i velikog broja sjemenki, najizglednije su u ekološkom optimumu vrste te bi se vjerojatno najlakše prilagodile budućim klimatskim promjenama. U skladu s tim ova populacija predstavlja evolucijski signifikantnu jedinicu koju treba uzeti u obzir u budućim programima zaštite.

Abstract (english)

In mountain regions, with increasing altitude, climate and ecological conditions change, which reflects in changes of vegetation composition. Since it can be assumed that widespread species will be less affected by climate change, than it is the case with narrowly distributed species, the study of species tolerant to altitude changes is particularly interesting in the light of today's climate change. The aim of this graduation thesis was to conduct a preliminary population genetic study of three natural populations of G. monvillei (Cactaceae) from different altitudes using AFLP (Amplified Fragment Lenght Polymorphism) markers. After comparing obtained results with the already existing results of morphological and physiological studies, a more extensive AFLP studies are going to be defined on a larger number of natural populations. The observed genetic diversity, DW index values (frequency down-weighted marker), PCoA (Principal Coordinate Analysis) and STRUCTURE analyses indicated long-time isolation of the population from the highest altitude and free gene flow in the two populations from lower altitudes. Due to the highest genetic diversity, globular seedlings, resistance to high temperatures and drought, large fruits and large numbers of seeds, the population at 1250 m a.s.l. probably represents an ecological optimum of the species and it would most likely be the easiest population to adapt to future climate changes. Therefore, this population can be recognized as an evolutionarily significant unit in future conservation programs.

• Keywords: molekularna ekologija; AFLP; kaktusi
• Keywords (english): molecular ecology; AFLP; cacti
• Language: croatian
• URN:NBN: urn:nbn:hr:217:985195
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-07-13 07:14:08