Učinak anestetika izoflurana i ionizirajućeg zračenja na primarna oštećenja DNA u miša

master's thesis

• Title (english): Effect of anesthetic Isoflurane and ionising radiation on primary DNA damage in mice
• Author: Dunja Šikić
• Mentor: Vesna Benković (mentor)
• Mentor: Mirta Milić (mentor)
• Committee member: Vesna Benković (predsjednik povjerenstva)
• Committee member: Renata Matoničkin Kepčija (član povjerenstva)
• Committee member: Renata Šoštarić (član povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-09-16, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Izofluran je inhalacijski anestetik koji se koristiti za indukciju i održavanje opće anestezije. Neka istraživanja pokazuju da uzrokuje genotoksične učinke. Ionizirajuće zračenje (IR) je zračenje dovoljne energije za ioniziranje atoma, a niže doze mogu dovesti do promjena u staničnoj proliferaciji i oštećenja DNA. Izofluran i IR se koriste u kliničkoj praksi, a njihovo međudjelovanje još nije dovoljno istraženo. Cilj ovog istraživanja bio je utvrditi učinak anestetika izoflurana i IR na vrijednosti primarnih oštećenja DNA u leukocitima periferne krvi, stanicama mozga, jetre i bubrega u Swiss albino miševa. Miševi su anestezirani izofluranom (1,7 vol%) tijekom 2 sata nakon čega su ozračeni X- zračenjem (1 Gy). Metodom alkalnog komet testa analizirani su parametri: dužina repa kometa, postotak DNA u repu kometa i repni moment. U vremenskim intervalima 0, 2, 6 i 24 sata od izlaganja anesteziji i zračenju procijenjen je popravak nastalih oštećenja DNA. Rezultati ovog istraživanja pokazali su potencijalni zaštitni učinak izoflurana, naročito u stanicama jetre i mozga, a kao moguća posljedica tog učinka, kombinirani tretman pokazao je manja oštećenja DNA u odnosu na tretman samim zračenjem. Stanični indeks popravka pokazao se nešto manjim prilikom kombiniranog tretmana u leukocitima i stanicama bubrega, a višim u stanicama jetre. Iako su potrebna daljnja istraživanja, ovaj rad pokazao je potencijalni antagonistički utjecaj izoflurana na oštećenja DNA uzrokovana nakon izlaganja IR-u.

Abstract (english)

Isoflurane is an inhalation anesthetic used for induction and maintenance of general anesthesia. Some researches suggest that it causes genotoxic effects. Ionizing radiation (IR) is radiation of energy high enough to cause atom ionization. IR can cause changes in cellular proliferation and DNA damage. Both isoflurane and IR are used in clinical practice, and their combined effects are not yet sufficiently studied. The goal of this study was to determine the effect of isoflurane and IR on primary DNA damage in peripheral blood leukocytes and kidney, liver, and brain cells in Swiss albino mice. The mice were anesthetized using isoflurane (1.7 vol%) for 2 hours, after which they were irradiated using X-rays (1Gy). Alkaline comet assay method was used to analyze following parameters: tail length, percentage of DNA in comet tail and tail moment. Cellular DNA repair was assessed 0, 2, 6, and 24 hours after anesthesia and irradiation exposure. The results demonstrated a potentially protective effect of isoflurane, especially in liver and brain cells. As a possible result of that protective effect, combined treatment showed less DNA damage in comparison to IR itself. Cellular DNA repair index was somewhat lower in combined treatment in leukocytes and kidney cells, and higher in liver cells. Although additional research is required, this study showed a potential antagonistic effect of isoflurane on DNA damage caused by IR.

• Keywords: izofluran; ionizirajuće zračenje; primarna oštećenja DNA; alkalni komet test; stanični indeks popravka primarnih oštećenja DNA
• Keywords (english): isoflurane; ionising radiation; primary DNA damage; alkaline comet assay; cellular DNA repair index
• Language: croatian
• URN:NBN: urn:nbn:hr:217:545806
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-09-16
• Created on: 2020-09-21 13:21:19