Spektrofotometrijsko i spektrofluorimetrijsko određivanje prazikvantela

master's thesis

• Title (english): Spectrophotometric and spectrofluorimetric determination of praziquantel
• Author: Tena Matijević
• Mentor: Nives Galić (mentor)
• Committee member: Nives Galić (predsjednik povjerenstva)
• Committee member: Ines Primožič (član povjerenstva)
• Committee member: Dubravka Matković-Čalogović (član povjerenstva)
• Committee member: Snežana Miljanić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2020-09-10, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Prazikvantel (PZQ) je antihelmintik koji prema biofarmaceutskom sustavu klasifikacije spada u klasu II (niska topljivost, visoka permeabilnost). Međutim, utvrđeno je da mu se topljivost u biorelevantnom mediju povećava ukoliko je u kompleksu s ciklodekstrinom. Utjecaj β-ciklodekstrina i tri njegova derivata (hidroksipropil-β-ciklodekstrin, sulfobutileter-β-ciklodekstrin te nasumično metiliran β-ciklodekstrin) na topljivost PZQ u vodi istražen je u sklopu ovog diplomskog rada. Prazikvantel je u vodi kvantitativno određen validiranim UV/Vis spektrofotometrijskim i spektrofluorimetrijskim metodama. Dobiveni rezultati su uspoređeni s onima iz diplomskog rada Petre Tagliaretti u kojem su analizirani identični sustavi, a količina PZQ je određena tekućinskom kromatografijom visoke djelotvornosti.

Abstract (english)

Praziquantel (PZQ) is an anthelmintic which is categorized as a class II compound (low solubility, high permeability) according to the Biopharmaceutical Classification System. However, its solubility in biorelevant media was found to increase if it was complexed by cyclodextrins. Effect of β-cyclodextrin and three of its derivatives (hydroxypropyl-β-cyclodextrin, sulfobutylether-β-cyclodextrin and randomly methylated β-cyclodextrin) on the solubility of PZQ in water was studied in this thesis. Praziquantel in water was quantitatively determined by validated UV/Vis spectrophotometric and spectrofluorimetric methods. The obtained results were compared with those presented in Petra Tagliaretti's thesis, in which identical systems were analyzed and the amount of PZQ was determined by high performance liquid chromatography.

• Keywords: ciklodekstrini; inkluzijski kompleksi; prazikvantel; topljivost; fluorescencijska spektroskopija; UV/Vis apsorpcijska spektroskopija
• Keywords (english): cyclodextrins; inclusion complexes; praziquantel; solubility. fluorescence spectroscopy; UV/Vis absorption spectroscopy
• Language: croatian
• URN:NBN: urn:nbn:hr:217:032373
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-09-10
• Created on: 2020-09-24 10:48:09