Regulacije proteina Plekhm1 fosforilacijom u autofagiji

master's thesis

• Title (english): Investigat the Regulation of Plekhm1 in autophagy by phosphorylation
• Author: Andrea Gubaš
• Mentor: Inga Marijanović (mentor)
• Committee member: Inga Marijanović (predsjednik povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-09-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Autofagija je proces uključen u membranski prijenos proteina, organela, patogena i različitih nakupina do lizosoma, za razgradnju. Autofagiju čini široki molekularni sustav reguliran brojnim molekularnim mehanizmima. Proces makroautofagije zahtijeva nastajanje dvostruko-membranskog sloja koji okružuje ciljani materijal. Sloj dvostruke membrane se zatvara, te se s vremenom spaja s lizosomom. Jedan od glavnih regulatornih proteina u autofagiji je “mammalian target of rapamycin”, odnosno mTOR. Ranija istraživanja su pokazala kako je protein “Pleckstrin homology domain-containing family M member 1”, kraće Plekhm1, uključen u autofagiju. Mutacija u genu Plekhm1 vodi do osteopetroze, bolesti koja utječe na resorpciju kostiju. Naši neobjavljeni rezultati pokazuju da bi Plekhm1 mogao biti povezan uz kasnije stadije autofagije, te da reagira s proteinima Rab7 i LC3, nekima od pokazatelja kasnijih stadija autofagije. U ovom radu pokušavamo dati kratak uvid u regulaciju Plekhm1 u autofagiji i interakciju istog proteina s različitim markerima autofagije.

Abstract (english)

Autophagy is a process involved in membrane trafficking of proteins, organelles, pathogens and aggregates to lysosomes for degradation. Autophagy consists of large molecular machinery, regulated by numerous mechanisms. The process of macroautophagy requires the de novo formation of a double membrane layer that surrounds the target material, seals and eventually fuses with the lysosome. One of the key regulators in autophagy is mammalian target of rapamycin, mTOR. Previous studies showed the involvement of a protein called Pleckstrin homology domain-containing family M member 1, Plekhm1 in osteopetrosis, a disease affecting bone resorbtion. Our unpublished data suggests that Plekhm1 is involved in later stages of autophagy. Also, our data suggests that Plekhm1 interacts with Rab7 and LC3 proteins, which are indicatiors of late phase autophagy. Here, we give a brief insight into Plekhm1 regulation and highlight novel interactions with proteins involved in autophagy.

• Keywords: makroautofagija; Plekhm1; mTOR; interakcije; phos-tag
• Keywords (english): macroautophagy; Plekhm1; mTOR; interactions; phos-tag
• Language: croatian
• URN:NBN: urn:nbn:hr:217:605025
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 08:11:34